Cell lines and methods for producing proteins
OwnerStephen Parker, Yashwant Deoinventors
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Abstract
The present invention relates to the isolation and enrichment of avian oviduct tumor cells to obtain sustainable cell lines that can be passaged multiple times in cell culture and can be used for long-term production of heterologous polypeptides at higher yields. The methods of the instant invention involve the manipulation and/or propagation of oviduct tumor cells derived from either wild-type or transgenic avians.
Claims52
An isolated avian oviduct tumor cell line comprising a nucleic acid encoding a heterologous protein, wherein the cell line is sustainable in culture for at least three or more passages.
An immortal cell line derived from a tubular gland cell comprising a nucleic acid encoding a heterologous protein.
A method of producing a heterologous protein comprising,
- contacting an avian oviduct tumor cell with a nucleic acid encoding a heterologous protein under conditions suitable for protein expression, wherein the tumor cell is sustainable in culture for at least three or more passages;
- and
- isolating the heterologous protein.
A method of producing a heterologous protein comprising:
- isolating oviduct tumor cells that expresses the protein, wherein the cell is sustainable in culture for at least three or more passages;
- culturing the tumor cells under conditions suitable for expression of the protein;
- and
- isolating the protein.
Dependencies are inferred from the claim text.