Rescue of canine distemper virus from cdna
AssigneeAmerican Cyanamid Company
InventorsChristopher Parks, Mohinderjit Sidhu, Pramila Walpita, Gerald Kovacs, Stephen Udem
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Abstract
This invention relates to a method for recombinantly producing via rescue canine distemper virus, a nonsegmented, negative-sense, single-stranded RNA virus, and immunogenic compositions formed therefrom. Additional embodiments relate to methods of producing the canine distemper virus as an attenuated and/or infectious viruses. The recombinant viruses can be prepared from cDNA clones, and, accordingly, viruses having defined changes, including nucleotide/polynucleotide deletions, insertions, substitutions and rearrangements, in the genome can be obtained.
Claims48
A method for producing a recombinant canine distemper virus comprising;
- in at least one host cell, conducting transfection or transformation, in media, of a rescue composition which comprises (i) a transcription vector comprising an isolated nucleic acid molecule which comprises a polynucleotide sequence encoding a genome or antigenome of canine distemper virus, or variant polynucleotide sequence thereof, and (ii) at least one expression vector which comprises one more isolated nucleic acid molecule(s) comprising a polynucleotide sequence encoding the trans-acting proteins (N, P and L) necessary for encapsidation, transcription and replication;
- under conditions sufficient to permit the co-expression of said vectors and the production of the recombinant virus.
An immunogenic composition comprising an isolated, recombinantly-produced canine distemper virus and a physiologically acceptable carrier.
A nucleic acid molecule comprising a polynucleotide sequence encoding a genome or antigenome of canine distemper virus.
A nucleic acid molecule comprising a polynucleotide sequence encoding one or more proteins of the canine distemper virus.
A plasmid comprising a polynucleotide sequence encoding a genome or antigenome of canine distemper virus.
A plasmid comprising a polynucleotide sequence encoding one or more proteins of the canine distemper virus.
A composition comprising an isolated, recombinantly-produced canine distemper virus and a physiologically acceptable carrier;
- wherein the canine distemper virus expresses at least one heterologous polynucleotide.
A nucleotide sequence comprising the sequence of a cDNA clone of a recombinant canine distemper virus.
A nucleotide sequence comprising the polynucleotide sequence of a cDNA clone of a recombinant canine distemper virus of FIG. 6 (SEQ ID NO. 2) or FIG. 7 (SEQ ID NO. 3).
Dependencies are inferred from the claim text.