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AssigneeValtion Teknillinen Tutkimuskeskus
InventorsKristiina Kruus, Laura-Leena Kiiskinen, Marjaana Ratto, Liisa Viikari, Markku Saloheimo
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The invention relates to a new laccase enzyme, which can be isolated from the strains of the Melanocarpus genus, the M. albomyces strain in particular. The pH optimum of the enzyme is within 5-8 and the enzyme works at a temperature of 30-80° C. The isoelectric point of the enzyme is about 4.0 as determined by isoelectric focusing and the molecular weight about 80 kDa, defined by SDS-PAGE. The enzyme is especially well suited to applications, wherein the pH and temperature conditions are high. The invention also relates to a gene that encodes laccase enzyme, and a laccase enzyme produced by recombinant technology.
A laccase enzyme, characterized in that it is isolatable from the strains of the Melanocarpus genus, and that the pH optimum of the enzyme is within 5-8 and the enzyme functions at pH 3-9 and at a temperature of 30-80° C.
An isolated and purified Melanocarpus laccase enzyme, preferably a Melanocarpus albomyces laccase enzyme the pH optimum of which is within 5-8 and the enzyme functions at pH 3-9 and at a temperature of 30-80° C.
An enzyme preparation, characterized in that it comprises the laccase of any one of the preceding claims as the main activity.
An enzyme preparation, characterized in that it comprises the laccase of any of the preceding claims and that the enzyme preparation comprises laccase more than what the strains of the Melanocarpus genus naturally produce into their culture solution.
A nucleic acid molecule that encodes a polypeptide that has laccase enzyme activity, characterized in that the nucleic acid molecule is selected from the group comprising:
The nucleic acid molecule that encodes a polypeptide that has laccase enzyme activity, characterized in that the amino acid sequence of the polypeptide shows at least 80% identity with the amino acid sequence of SEQ ID NO:2 or FIG. 15.
A polypeptide, which has laccase activity, characterized in that it is encoded by a nucleic acid molecule, which is selected from the group comprising:
A polypeptide having laccase activity, characterized in that it comprises an amino acid sequence which shows at least 80% identity with the amino acid sequence of SEQ ID NO:2 or FIG. 15.
Dependencies are inferred from the claim text.