Nucleotide sequences coding for the lysRI gene

A process for producing L-amino acids comprising culturing a recombinant bacterial cell in a medium suitable for producing L-amino acids, and collecting the L-amino acids produced wherein said bacterial cell comprises an attenuated lysR1 gene.

A process for screening for polynucleotides which encode a protein having LysR1 transcriptional regulatory activity comprising hybridizing the isolated polynucleotide which encodes a protein comprising the amino acid sequence of SEQ ID NO:2 to the polynucleotide to be screened;

• expressing the polynucleotide to produce a protein;
• and detecting the presence or absence of LysR1 transcriptional regulatory activity in said protein.

A process for screening for polynucleotides which encode a protein having LysR1 transcriptional regulatory activity comprising hybridizing isolated polynucleotide comprising SEQ ID NO:

• 1 to the polynucleotide to be screened;
• expressing the polynucleotide to produce a protein;
• and detecting the presence or absence of LysR1 transcriptional regulatory activity in said protein.

A method for detecting a nucleic acid with at least 70% homology to an isolated polynucleotide which encodes a protein comprising SEQ ID NO:2, comprising contacting a nucleic acid sample with a probe or primer comprising at least 15 consecutive nucleotides of said isolated polvnucleotide, or at least 15 consecutive nucleotides of the complement thereof.

A method for producing a nucleic acid with at least 70% homology to an isolated polynucleotide which encodes a protein comprising SEQ ID NO:2, comprising contacting a nucleic acid sample with a primer comprising at least 15 consecutive nucleotides of the isolated polynucleotide, or at least 15 consecutive nucleotides of the complement thereof.

A method for detecting a nucleic acid with at least 70% homology to isolated polynucleotide comprising SEQ ID NO:

• 1, comprising contacting a nucleic acid sample with a probe or primer comprising at least 15 consecutive nucleotides of the nucleotide sequence of said isolated polynucleotide, or at least 15 consecutive nucleotides of the complement thereof.

A method for producing a nucleic acid with at least 70% homology to comprising contacting a nucleic acid sample with a primer comprising at least 15 consecutive nucleotides of the isolated polynucleotide, or at least 15 consecutive nucleotides of the complement thereof.

37-38. (canceled)

An isolated polypeptide comprising the amino acid sequence of SEQ ID NO:2.