Fermentative production of d-hydroxyphenylglycine and d-phenylglycine
OwnerCraig Townsend, Michele Gunsior +3inventors
InventorsCraig Townsend, Michele Gunsior, Ulrike Muller, Friso Assema Van, Theodorus Sonke
Life of this patent
Estimating the term…
Abstract
A new fermentative process for the preparation of D-p-hydroxyphenylglycine (D-HPG) or D-henylglycine (D-pG) in enantiomerically pure form is disclosed. Precursors for the formation of D-HPG and D-pG are withdrawn form the common aromatic amino acid pathway, converted to p-hydroxyphenylglyoxylate or phenylglyoxylate, and are finally converted to D-HPG or D-pG by the action of a stero-inverting D-aminotransferase.
Claims10
A fermentative process in which a recombinant microorganism produces a D-phenylglycine product, in particular D-phenylglycine (D-pG) or D-p-hydroxyphenylglycine (D-HPG), wherein
- a) for the production of D-pG, respectively for the production of D-HPG, phenylpyruvate (PP), respectively p-hydroxyphenylpyruvate (HPP) is withdrawn from the aromatic amino acid pathway
- b) and is converted to mandelic acid (MA) or p-hydroxymandelic acid (HMA), respectively,
- c) thereafter being converted into phenylglyoxylate or p-hydroxyphenylglyoxylate, respectively,
- d) and the phenylglyoxylate or p-hydroxyphenylglyoxylate thereafter being converted into D-phenylglycine (D-pG) or D-p-hydroxyphenylglycine (D-HPG) by the action of a stereo-inverting D-aminotransferase, respectively.
A recombinant cell which is capable of secreting detectible amounts of D-HPG or D-pG and which contains
- a) genes coding for enzymes which catalyze the conversion of p-hydroxyphenylpyruvate (HPP) to p-hydroxymandelic acid (HMA) or phenylpyruvate (PP) to mandelic acid (MA)
- b) genes coding for enzymes which catalyze the conversion of p-hydroxymandelic acid (HMA) to p-hydroxyphenylglyoxylate or of mandelic acid (MA) to phenylglyoxylate
- c) and genes coding for a stereo-inverting D-aminotransferase which catalyzes the conversion of p-hydroxyphenylglyoxylate to D-HPG or phenylglyoxylate to D-pG.
Dependencies are inferred from the claim text.