Mutant tyrosine repressor gene and utilization thereof
AssigneeAjinomoto Co., Inc.
InventorsHidehiko Kumagai, Hideyuki Suzuki, Takane Katayama
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Abstract
A gene coding for a mutant tyrosine repressor having increased positive regulatory activity for expression of the tyrosine phenol lyase gene compared with a tyrosine repressor not having mutation is obtained by introducing a mutation into a tyrosine repressor gene of Erwinia herbicola, introducing the gene into which the mutation is introduced into Escherichia coli expressing a lactose operon under the control of a promoter and enhancer of the tyrosine phenol lyase gene derived from Erwinia herbicola, and selecting a transformed strain having increased β-galactosidase activity.
Claims24
1-16. (canceled)
A DNA coding for the mutant tyrosine repressor having at least one or more mutations selected from replacement of valine residue at a position of 67 with an amino acid residue other than valine residue, replacement of tyrosine residue at a position of 72 with an amino acid residue other than tyrosine residue, replacement of aspartic acid residue at a position of 97 with an amino acid residue other than aspartic acid residue and replacement of isoleucine residue at a position of 402 with an amino acid residue other than isoleucine residue and having an increased positive regulatory activity for expression of tyrosine phenol lyase gene compared with a tyrosine repressor not having those mutations.
Dependencies are inferred from the claim text.