Chemically modified enzymes with multiple charged variants
AssigneesGenencor International, Inc., The Governing Council of the University of Toronto
InventorsBenjamin David, John Jones, Richard Bott
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Abstract
This invention provides modified enzymes comprising one or more amino acid residues replaced by cysteine residues, where the cysteine residues are modified by replacing the thiol hydrogen in the cysteine residues with a substituent group providing a thiol side chain comprising a multiply charged moiety. The enzymes show improved interaction and/or specificity and/or activity with charged substrates.
Claims11
1-35. canceled
A method for assaying a chemically modified mutant enzyme, the modified mutant enzyme being a chemically modified mutant of a protease, Bacillus lentus subtilisin, obtained by replacement of an amino acid residue with various alkyl and mono, di- and triammonium groups using methanethiosulfonate, to determine the degree of removal of a protein stain from a material, said method comprising:
- a. providing a swatch of material comprising a piece of material and a protein stain;
- b. fixing the protein stain to the material with a cross-linking agent;
- c. applying a chemically modified mutant enzyme to the swatch;
- d. incubating the swatch and the chemically modified mutant enzyme to determine the degree of removal of the protein stain from the material and
- e. washing the incubated swatch.
38 and 39. (canceled)
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46-70. (canceled).
Dependencies are inferred from the claim text.