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US 2005/0089964 A1Published application
Nucleic acid molecules encoding red and green emitting luciferases
AssigneeToyo Boseki Kabushiki Kaisha
InventorsVadim Viviani, Yoshihiro Ohmiya
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Abstract
Isolated nucleic acid molecules which code for luciferases able to produce the green bioluminescence of Phrixotrhix vivianii and red bioluminescence of Phrixothrix hirtus are described. The nucleic acid molecules and the luciferases encoded thereby can be used in applications such as diagnostic methods and molecular biology tools.
Claims3
An isolated nucleic acid molecule encoding a red light emitting luciferase which hybridizes under high stringency conditions to a nucleotide sequence selected from the group consisting of:
- (a) SEQ ID NO.3, and
- (b) the complement of SEQ ID No.3,
- wherein said high stringency conditions correspond to washing in prewarmed (68° C.) solution containing 0.1×SSC/0.1% SDS for 15 min at 68° C.,
- wherein the encoded red light emitting luciferase emits red bioluminescence having a maximum λ of approximately 622 nm when expressed in E coli.
1 more element
Dependencies are inferred from the claim text.