Novel Bacillus thuringiensis strain, crystal gene and crystal protein and uses thereof
AssigneeAgriculture Agroalimentaire Canada
InventorsJean-Charles Cote, Yong-Chul Jung, Eiichi Mizuki, Tetsuyuki Akao
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Abstract
A novel Bacillus thuringiensis strain deposited at the International Depository Authority of Health Canada in Winnipeg under accession number IDAC010201-5, its crystal gene having the sequence SEQ ID NO: 1 and crystal protein encoded by same having the sequence SEQ ID NO: 2 and uses thereof. More specifically, the present invention is concerned with a novel Bacillus thuringiensis, novel cry31 protoxin and toxin, nucleotide sequences encoding same and anti-cancer therapeutic applications for the toxin.
Claims15
A biologically pure culture of a microorganism strain comprising all of the identifying characteristics of a Bacillus thuringiensis strain deposited at the International Depository Authority of Health Canada in Winnipeg under accession number IDAC010201-5, or a mutant thereof derived from said strain.
An isolated nucleic acid molecule comprising a polynucleotide sequence selected from the group consisting of:
- (a) a nucleotide sequence encoding a polypeptide comprising the complete amino acid sequence in SEQ ID NO:
- 2;
- (b) a nucleotide sequence encoding a polypeptide comprising the complete amino acid sequence in SEQ ID NO:
- 8;
- (c) a nucleotide sequence encoding a polypeptide comprising the complete amino acid sequence in SEQ ID NO:
- 12, with the proviso that said nucleotide sequence does not encode the amino acid sequence in SEQ ID NO:
- 18;
- (d) a nucleotide sequence encoding a polypeptide comprising the complete amino acid sequence in SEQ ID NO:
- 13, with the proviso that said nucleotide sequence does not encode the amino acid sequence at positions 232 to 723 of SEQ ID NO:
- 18;
- (e) a nucleotide sequence encoding a polypeptide comprising the complete amino acid sequence in SEQ ID NO:
- 14, with the proviso that said nucleotide sequence does not encode the amino acid sequence in SEQ ID NO:
- 18;
- (f) a nucleotide sequence encoding a polypeptide comprising the complete amino acid sequence in SEQ ID NO:
- 15, with the proviso that said nucleotide sequence does not encode the amino acid sequence at positions 232 to 723 of SEQ ID NO:
- 18;
- (g) a nucleotide sequence encoding a polypeptide comprising the complete amino acid sequence of a crystal protein contained in the Bacillus thuringiensis strain deposited at the International Depository Authority of Health Canada in Winnipeg under accession number IDAC010201-5;
- (h) a nucleotide sequence encoding a crystal protein comprising the complete amino acid sequence in SEQ ID NO:
- 10;
- (i) a nucleotide sequence comprising the sequence set forth in SEQ ID NO:1;
- (j) a nucleotide sequence comprising the sequence set forth in SEQ ID NO:
- 9;
- (k) a nucleotide sequence encoding a crystal protein comprising the sequence set forth in SEQ ID NO:
- 11;
- (l) a nucleotide sequence encoding a crystal protein having at least 94% identity with the complete amino acid sequence in SEQ ID NO:
- 2, with the proviso that said nucleotide sequence does not encode the amino acid sequence in SEQ ID NO:
- 18;
- (m) a nucleotide sequence encoding a crystal protein having at least 97% identity with the complete amino acid sequence in SEQ ID NO:
- 8 with the proviso that said nucleotide sequence does not encode the amino acid sequence from position 232 to 723 of SEQ ID NO:
- 18;
- (n) a nucleotide sequence encoding a crystal protein cytotoxic against at least one human cancer cell, said nucleotide sequence having at least 98% identity with the complete sequence set forth in SEQ ID NO:
- 9, with the proviso that said nucleotide sequence does not encode the amino acid sequence from position 232 to 723 of SEQ ID NO:
- 18;
- (o) a nucleotide sequence completely complementary to any of the nucleotide sequences in (a), (b), (c), (d), (e), (f), (g), (h), (i) (j), (k), (l), (m) and (n);
- and
- (p) a nucleotide sequence which hybridizes under high stringency conditions to any of the nucleotide sequences in (a), (b), (c), (d), (e), (f), (g), (h), (i) (j), (k), (l), (m), (n) and (o).
An isolated polypeptide comprising a sequence selected from the group consisting of:
- (a) an amino acid as set forth in SEQ ID NO:
- 2;
- (b) an amino acid sequence in SEQ ID NO:
- 8;
- (c) an amino acid sequence of a crystal protein contained in the bacillus thuringiensis strain in the deposit at the International Depository Authority of Health Canada in Winnipeg under accession number IDAC010201-5;
- (d) a crystal protein comprising the amino acid sequence in SEQ ID NO:
- 10;
- (e) a crystal protein having at least 94% identity with the complete amino acid sequence in SEQ ID NO:
- 2, with the proviso that said crystal protein is not constituted of SEQ ID NO:
- 18;
- (f) a crystal protein having at least 97% identity with the complete amino acid sequence in SEQ ID NO:
- 8, with the proviso that said crystal protein is not constituted the amino acid sequence at positions 232 to 723 of SEQ ID NO:
- 18;
- (g) a crystal protein cytotoxic against at least one human cancer cell and encoded by a nucleotide sequence having at least 98% identity with the complete sequence in SEQ ID NO:
- 9, with the proviso that said nucleotide sequence does encode the amino acid sequence at positions 232 to 723 of SEQ ID NO:
- 18.
A method of modulating the level of cry31Aa2 active protein in a cell comprising a modulation of the level or activity of the sequence SEQ ID NO:
- 8.
Dependencies are inferred from the claim text.