Process for producing alpha 2,3/ alpha 2,8-sialyltransferase and sialic acid-containing complex sugar
AssigneeKyowa Hakko Kogyo Co., Ltd.
InventorsTetsuo Endo, Satoshi Koizumi
Life of this patent
Estimating the term…
Abstract
The present invention can provide a process for producing a protein having α2,3/α2,8-sialyltransferase activity using a transformant comprising a DNA encoding a protein having α2,3/α2,8-sialyltransferase activity derived from a microorganism belonging to the genus Pasteurella and a process for producing a sialic acid-containing complex carbohydrate using a transformant capable of producing a protein having α2,3/α2,8-sialyltransferase activity derived from a microorganism.
Claims29
A process for producing a protein having α2,3/α2,8-sialyltransferase activity, which comprises:
- culturing a transformant capable of producing a protein having α2,3/α2,8-sialyltransferase activity derived from a microorganism belonging to the genus Pasteurella in a medium to produce and accumulate the protein having α2,3/α2,8-sialyltransferase activity in the culture, and recovering the protein from the culture.
A process for producing a sialic acid-containing complex carbohydrate, which comprises:
- allowing a culture of a transformant capable of producing a protein having α2,3/α2,8-sialyltransferase activity derived from a microorganism or a treated product of the culture as an enzyme source, cytidine-5′-monophosphosialic acid and an acceptor complex carbohydrate to be present in an aqueous medium to produce and accumulate the sialic acid-containing complex carbohydrate in the aqueous medium, and recovering the sialic acid-containing complex carbohydrate from the aqueous medium.
A protein having α2,3/α2,8-sialyltransferase activity, which comprises the amino acid sequence represented by SEQ ID NO:1.
A protein having α2,3/α2,8-sialyltransferase activity, which comprises the amino acid sequence represented by SEQ ID NO:3.
A protein having α2,3/α2,8-sialyltransferase activity, which comprises the amino acid sequence represented by SEQ ID NO:5.
A protein which consists of an amino acid sequence in which at least one amino acid is deleted, substituted or added in the amino acid sequence represented by any one selected from SEQ ID NOs:1, 3 and 5, and has α2,3/α2,8-sialyltransferase activity.
A protein which has an identity of at least 80% with the amino acid sequence represented by any one selected from SEQ ID NOs:1, 3 and 5, and has α2,3/α2,8-sialyltransferase activity.
Dependencies are inferred from the claim text.