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US 2005/0089930 A1Published application
Polypeptide fingerprinting methods
AssigneeTarget Discovery, Inc.
InventorsLuke Schneider, Michael Hall, Robert Petesch, Jeffrey Peterson
Life of this patent
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Abstract
The invention provides methods, compositions, apparatus, and a computer data retrieval system for conducting proteomics.
Claims10
1-21. (canceled)
A method of generating from a sample a high-resolution protein expression fingerprint dataset containing a plurality of polypeptide sequence tags, comprising:
- (i) obtaining a protein-containing extract from said sample, wherein said sample comprises a plurality of intact proteins;
- (ii) electrophoresing said extract in a multidimensional capillary electrophoresis device having an electroosmotic flow of less than 1×10−4 cm2 V-s to separate and elute intact protein species to resolve said intact protein species based on at least one first biophysical parameter which discriminates protein species;
- (iii) obtaining, by mass spectrographic fragmentation of a plurality of eluted intact protein species, a plurality of polypeptide sequence tags;
- (iv) generating a high-resolution protein expression fingerprint dataset containing said plurality of polypeptide sequence tags.
1 more element
The method of claim 1, wherein the method further comprises, prior to step (iii):
- (iia) electrophoresing eluted intact protein species eluted from said multidimensional capillary electrophoresis device in a second capillary electrophoresis device, or plurality thereof in parallel, to separate and elute intact protein species thereby resolving said protein species based on at least one second biophysical parameter which discriminates protein species.
Dependencies are inferred from the claim text.