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US 2005/0089918 A1Published application
In-situ cell extraction and assay method
AssigneeAmersham Biosciences UK Limited
InventorJeffrey Horton
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Abstract
This invention provides a simple and convenient, single stage, single vessel cell extraction and assay method which is suitable for the extraction and measurement of a range of different types of analyte which occur as cellular components. The invention also provides kits of reagents suitable for performing cellular extraction and measurement as a single stage, single vessel process.
Claims14
A method of assaying for an analyte which method comprises the steps of:
- i) mixing a sample of cells possibly containing the analyte with a cell lysis reagent to provide a cell lysis fluid,
- ii) mixing the cell lysis fluid with reagents, including a specific binding partner of the analyte for binding to the analyte, for performing a specific binding assay for the analyte,
- iii) and mixing the cell lysis fluid with a sequestrant for the cell lysis reagent, whereby the binding of step ii) is performed in the presence of the sequestrant.
Dependencies are inferred from the claim text.