Alternatively spliced isoforms of nuclear factor kappa-B, subunit 1 (NFKB1)

A method for screening for a compound able to bind to NFKB1sv2 comprising the steps of:

• (a) expressing a polypeptide comprising SEQ ID NO 6 from recombinant nucleic acid;
• (b) providing to said polypeptide a test preparation comprising one or more test compounds;
• and
• (c) measuring the ability of said test preparation to bind to said polypeptide.

A method of screening for compounds able to bind selectively to NFKB1sv2 comprising the steps of:

• (a) providing a NFKB1sv2 polypeptide comprising SEQ ID NO 6;
• (b) providing one or more NFKB1 isoform polypeptides that are not NFKB1sv2;
• (c) contacting said NFKB1sv2 polypeptide and said NFKB1 isoform polypeptide that is not NFKB1sv2 with a test preparation comprising one or more compounds;
• and
• (d) determining the binding of said test preparation to said NFKB1sv2 polypeptide and to said NFKB1 isoform polypeptide that is not NFKB1sv2, wherein a test preparation that binds to said NFKB1sv2 polypeptide, but does not bind to said NFKB1 polypeptide that is not NFKB1sv2, contains a compound that selectively binds said NFKB1sv2 polypeptide.

A method for screening for a compound able to bind to or interact with a NFKB1sv2 protein or a fragment thereof comprising the steps of:

• (a) expressing a NFKB1sv2 polypeptide comprising SEQ ID NO 6 or fragment thereof from a recombinant nucleic acid;
• (b) providing to said polypeptide a labeled NFKB1 ligand that binds to said polypeptide and a test preparation comprising one or more compounds;
• and
• (c) measuring the effect of said test preparation on binding of said labeled NFKB1 ligand to said polypeptide, wherein a test preparation that alters the binding of said labeled NFKB1 ligand to said polypeptide contains a compound that binds to or interacts with said polypeptide.