Variants of cyan fluorescent protein with improved fluorescent properties
AssigneeVanderbilt University
InventorsDavid Piston, Mark Rizzo
Life of this patent
Estimating the term…
Abstract
The present invention provide optimized cyan fluorescent protein (oCFP) variants. In particular, the variants exhibit increase quantum yield of fluorescence and a single component fluorescence lifetime.
Claims40
An optimized variant of cyan fluorescent protein comprising an amino acid other than Y at position 145.
An optimized variant of cyan fluorescent protein comprising a non-hydrophobic residue at position 148.
A nucleic acid encoding an optimized variant of cyan fluorescent protein comprising an amino acid other than Y at position 145.
A nucleic acid encoding an optimized variant of cyan fluorescent protein comprising a non-hydrophobic residue at position 148.
A host cell comprising a nucleic acid encoding an optimized variant of cyan fluorescent protein comprising an amino acid other than Y at position 145 and/or a non-hydrophobic residue at position 148.
A method of assessing fluorescence resonance energy transfer (FRET) comprising:
- (a) bringing into FRET proximity an acceptor fluorescent protein (AFP) and an optimized variant of cyan fluorescent protein (oCFP), wherein said oCFP comprises an amino acid other than Y at position 145 and/or a non-hydrophobic residue at position 148;
- and
- (b) measuring FRET.
Dependencies are inferred from the claim text.