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AssigneeIowa State University Research Foundation, Inc.
InventorsMarc Porter, Jing Ni, Robert Lipert, G. Dawson
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The present invention provides a new class of Raman-active reagents for use in biological and other applications, as well as methods and kits for their use and manufacture. Each reagent includes a Raman-active reporter molecule, a binding molecule, and a surface enhancing particle capable of causing surface enhanced Raman scattering (SERS). The Raman-active reporter molecule and the binding molecule are affixed to the particle to give both a strong SERS signal and to provide biological functionality, i.e. antigen or drug recognition. The Raman-active reagents can function as an alternative to fluorescence-labeled reagents, with advantages in detection including signal stability, sensitivity, and the ability to simultaneously detect several biological materials. The Raman-active reagents also have a wide range of applications, especially in clinical fields (e.g., immunoassays, imaging, and drug screening).
A Raman-active reagent comprising a Raman-active reporter molecule which includes a reactive group, a binding molecule, and a surface enhancing particle capable of causing surface enhanced Raman scattering, the Raman-active reporter molecule being chemically linked to the surface enhancing particle and providing a detectable or measurable Raman scattering signal when illuminated by an excitation source capable of inducing a Raman scattering, the reactive group operably linked to the binding molecule, and wherein binding molecule is capable of specifically binding to a target analyte.
A Raman-active reagent comprising a Raman-active reporter molecule which includes a reactive group, a binding molecule, and a surface enhancing particle being a colloidal gold particle capable of causing surface enhanced Raman scattering, the Raman-active reporter molecule being 5,5′-dithiobis(succinimidyl-2-nitrobenzoate) linked to the surface enhancing particle by a thiol linkage and providing a detectable or measurable Raman scattering signal when illuminated by an excitation source capable of inducing a Raman scattering, the reactive group being the succinimide of the disthiobis(succinimidyl-2-nitrobenzoate), and wherein binding molecule is an antibody capable of specifically binding to a target analyte
A method for determining the presence or amount of a target analyte in a test sample, the method comprising the steps of:
A kit for determining the presence or amount of a target analyte in a test sample, the kit comprising
Dependencies are inferred from the claim text.