Base sequence detecting electrode, base sequence detecting device and base sequence detecting method

A base sequence detecting device comprising:

• a conductive detecting electrode;
• first blocking molecules formed so as to cover a surface of the detecting electrode, the first blocking molecules decreasing adsorption of an intercalating agent to the surface of the detecting electrode;
• a target-complementary probe immobilized to the detecting electrode, the target-complementary probe including a base sequence complementary to a target base sequence which is an object of detection;
• a conductive comparison electrode;
• second blocking molecules formed so as to cover a surface of the comparison electrode, the second blocking molecules decreasing adsorption of an intercalating agent to the surface of the comparison electrode;
• and
• a subtracter which subtracts an electrochemical signal detected at the comparison electrode from an electrochemical signal detected at the detecting electrode.

A base sequence detecting method comprising:

• detecting electrochemical signals at a detecting electrode and a comparison electrode of a base sequence detecting device comprising:
• the conductive detecting electrode;
• first blocking molecules formed so as to cover a surface of the detecting electrode,
• the first blocking molecules decreasing adsorption of an intercalating agent to the surface of the detecting electrode;
• a target-complementary probe immobilized to the detecting electrode via a first spacer member comprising straight chain organic molecules, the target-complementary probe including a base sequence complementary to a target base sequence which is an object of detection;
• the conductive comparison electrode;
• and second blocking molecules formed so as to cover a surface of the comparison electrode,
• the second blocking molecules decreasing adsorption of an intercalating agent to the surface of the comparison electrode;
• and
• subtracting an electrochemical signal detected at the comparison electrode from an electrochemical signal detected at the detecting electrode.