Desalting plate for maldi mass spectrometry
AssigneeWaters Investments Limited
InventorsEdouard Bouvier, Bruce Compton, Dominic Gostick, Jeffrey Brown
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Abstract
A novel Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry (MALDI-MS) desalting sample support plate is described. The plate comprises a sample presentation surface, wherein the sample presentation surface comprises at least one receiving surface of an absorbent layer, wherein the absorbent layer retains selected molecules on the receiving surface. Methods for making and using the sample support plate in conventional and automated MALDI-MS are also described.
Claims50
A desalting sample support plate, for use in Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry (MALDI-MS), comprising a sample presentation surface, wherein the sample presentation surface comprises at least one receiving surface of an adsorbent layer, wherein the adsorbent layer retains selected molecules on the receiving surface.
A method for performing Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry (MALDI-MS) on an analyte of interest, comprising:
- providing a sample support plate comprising a sample presentation surface, wherein the sample presentation surface comprises at least one receiving surface of an adsorbent layer, wherein the adsorbent layer retains selected molecules on the receiving surface;
- applying a sample comprising an analyte of interest to the receiving surface of the adsorbent layer;
- allowing the analyte of interest in the sample to be retained on the adsorbent layer;
- washing the adsorbent layer with an aqueous solution to remove the salts from the adsorbent layer, thereby desalting the sample;
- and
- performing MALDI-MS on the desalted analyte of interest retained on the adsorbent layer.
Dependencies are inferred from the claim text.