Novel column packing material
AssigneeSequant AB
InventorsWen Jiang, Knut Irgum
Life of this patent
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Abstract
A porous silica particle suitable can be used in zwitterionic high performance liquid chromatography. The particle comprises covalently bound zwitterionic groups grafted through a polymerization reaction. A hydrophilic column packing material suitable for use as a stationary phase in zwitterionic high performance liquid chromatography may comprise porous silica particles. The particles may comprise covalently bound zwitterionic groups. Methods for manufacturing and applying the silica particles exist.
Claims14
A porous silica particle for use in zwitterionic and hydrophilic interaction high performance liquid chromatography, comprising covalently bound zwitterionic groups.
A column packing material suitable for use as a stationary phase in zwitterionic and hydrophilic interaction high performance liquid chromatography, comprising porous silica particles, wherein said porous silica particles comprise covalently bound zwitterionic groups.
A method for producing porous silica particles for zwitterionic and hydrophilic interaction high performance liquid chromatography, comprising:
- providing porous silica particles suitable for use in zwitterionic and hydrophilic interaction high performance liquid chromatography;
- reacting said silica particles with thionyl chloride, thereby obtaining activated silica particles;
- reacting said activated silica particles with a tert-(C4-C10)-alkyl hydroperoxide to couple said tert-(C4-C10)-alkyl hydroperoxide to said activated silica particles, thereby obtaining peroxide-functionalized silica particles;
- and
- adding a zwitterionic methacryloxyethyl monomer to said peroxide-functionalized silica particles, thereby initiating graft polymerization of said zwitterionic methacryloxyethyl monomer to said peroxide-functionalized silica particles, wherein
- the porous silica particles comprise covalently bound zwitterionic groups.
A method for producing porous silica particles for zwitterionic and hydrophilic interaction high performance liquid chromatography, comprising:
- providing porous silica particles suitable for use in zwitterionic and hydrophilic interaction high performance liquid chromatography;
- suspending the silica particles in an aqueous solution of a zwitterionic methacryloxyethyl monomer, thereby obtaining a suspension of silica particles;
- allowing said suspension to equilibrate at room temperature;
- heating said equilibrated suspension to a temperature at or between 40° C. and 70° C.;
- and
- adding an aqueous solution of ammonium cerium nitrate, thereby initiating polymerization, wherein
- the porous silica particles comprise covalently bound zwitterionic groups.
Dependencies are inferred from the claim text.