Continuous acidification demineralization process for producing osteoinductive bone; and osteoinductive bone produced thereby

A rapid demineralization process for producing osteoinductive bone, comprising:

• subjecting bone to a continuous exchange of a predetermined volume of a demineralizing acid solution under conditions effective to produce demineralized bone.

A rapid demineralization process for producing osteoinductive demineralized bone, comprising:

• placing an amount of bone to be demineralized into a substantially closed container;
• and
• recirculating a predetermined volume of demineralizing acid solution from a said substantially closed container through an ion exchange media to remove calcium and phosphate thereby producing a regenerated acid, and returning said regenerated acid to said substantially closed container to produce said osteoinductive demineralized bone.

The rapid demineralization process of claim 8, said bone is demineralized at a rate of greater than 10.0 g/min.

The rapid demineralization process of claim 9, said bone is demineralized at a rate of grater than 15.0 g/min.

The rapid demineralization process of claim 10, said bone is demineralized at a rate of grater than 20.0 g/min.

The rapid demineralization process of claim 12, said agitation comprising stirring.

The rapid demineralization process of claim 20, said stirring comprising stirring at from about 1200 rpm to about 1500 rpm.

The rapid demineralization process of claim 21, said stirring comprising stirring at from about 1200 rpm to about 1500 rpm.

The rapid demineralization process of claim 24, said orbital shaking comprising orbital shaking at a rate of from about 30 cycles/min. to about 50 cycles/min.

The rapid demineralization process of claim 25, said orbital shaking comprising orbital shaking at a rate of from about 30 cycles/min. to about 50 cycles/min.

The rapid demineralization process of claim 28, said process being carried out at a temperature of from about 15° C. to about 50° C.

The rapid demineralization process of claim 29, said process being carried out at a temperature of from about 20° C. to about 40° C.

The rapid demineralization process of claim 32, said predetermined volume comprising 8.0 liters or less.

The rapid demineralization process of claim 33, said predetermined volume comprising 6.0 liters or less.

The rapid demineralization process of claim 34, said predetermined volume comprises 4.0 liters or less.

A rapid demineralization process for demineralizing bone, comprising:

• subjecting bone to a continuous exchange of a demineralized acid solution, where said bone is demineralized until a predetermined residual calcium level in said bone is achieved.

A rapid demineralization process for producing osteoinductive bone, comprising:

• subjecting bone to a continuous exchange of a predetermined volume of a demineralizing acid solution to produce demineralized bone, where calcium and phosphate are continually removed from said demineralizing acid solution.

A rapid demineralization process for producing osteoinductive bone, comprising:

• subjecting bone to a continuous exchange of a predetermined volume of a demineralizing acid solution under conditions effective to produce demineralized bone, where calcium and phosphate are continually removed from said demineralizing acid solution.

A rapid demineralization process for producing osteoinductive bone, comprising:

• subjecting bone to a continuous flow of a predetermined volume of a demineralizing acid solution maintained at a constant concentration, to produce demineralized bone.