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US 2005/0032207 A1Published application
Method for isolating, culturing and differentiating intestinal stem cells for therapeutic use
OwnerAnna Wobus, Alexandra Rolletschek +3inventors
InventorsAnna Wobus, Alexandra Rolletschek, Cornelia Wiese, Blyszczuk Przemyslaw, St-Onge Luc
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Abstract
The present invention relates to methods for the isolation, culture, and production of undifferentiated somatic intestinal stem/progenitor cells of mammalian, preferably human origins. The resulting stem/progenitor cells resemble properties of embryonic stem (ES) and multipotent progenitor cells with respect to morphology, biochemical property, and in pluripotency.
Claims37
A method of isolating and cultivating undifferentiated somatic intestinal stem or progenitor cells comprising:
- (a) isolating intestinal stem or progenitor cells from an intestinal epithelium of mammalian original, and
- (b) cultivating said intestinal stem cells on feeder cells under culture conditions allowing growth of said intestinal stem cells in an undifferentiated state.
Dependencies are inferred from the claim text.