Method for production of S-hydroxynitrile lyase by use of escherichia coli
AssigneeNippon Shokubai Co., Ltd.
InventorsHisashi Semba, Eita Ichige, Masaharu Mukouyama
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Abstract
A method for efficient production of an S-hydroxynitrile lyase by gene recombination using Escherichia coli is provided. A gene is formed by altering a codon in an S-hydroxyniitrylase gene originating in cassava (Manihot esculenta Crantz) without changing the amino acid sequence thereof till the frequency of codon usage wholly with an amino acid in Escherichia coli reaches a level of not less than 5%. According to the method for the production of an S-hydroxynitrile lyase by the use of the gene, the S-hydroxynitrile lyase can be produced in a large amount with an unusually high yield.
Claims14
A gene formed by altering a codon in an S-hydroxynitrile lyase gene originating in cassava (Manihot esculenta Crantz) without changing the amino acid sequence thereof till the frequency of use of the codon wholly with the amino acid in Escherichia coli reaches a level of not less than 5%.
A DNA formed of the base sequence of the sequence number 2.
Dependencies are inferred from the claim text.