US 2005/0032166 A1 · Polynucleotides encoding novel adiponectin receptor variants

Abstract

The present invention provides novel polynucleotides encoding human AdipoR2v1 polypeptides, mouse AdipoR2v1 polypeptides, human AdipoR3 polypeptides, human AdipoR2v2 polypeptides, human AdipoR3v1 polypeptides, rat AdipoR1 polypeptides, rat AdipoR2 polypeptides, fragments and homologues thereof. Also provided are vectors, host cells, antibodies, and recombinant and synthetic methods for producing said polypeptides. The invention further relates to diagnostic and therapeutic methods for applying these novel human AdipoR2v1 polypeptides, mouse AdipoR2v1 polypeptides, human AdipoR3 polypeptides, human AdipoR2v2 polypeptides, human AdipoR3v1 polypeptides, rat AdipoR1 polypeptides, rat AdipoR2 polypeptides, to the diagnosis, treatment, and/or prevention of various diseases and/or disorders related to these polypeptides. The invention further relates to screening methods for identifying agonists and antagonists of the polynucleotides and polypeptides of the present invention.

An isolated nucleic acid molecule comprising a polynucleotide having a nucleotide sequence selected from the group consisting of:

(a) a polynucleotide of SEQ ID NO:1 or a polynucleotide of the cDNA sequence included in ATCC Deposit No:
XXXXX, which is hybridizable to SEQ ID NO:1;
(b) a polynucleotide encoding a polypeptide of SEQ ID NO:2 or a polypeptide encoded by the cDNA sequence included in ATCC Deposit No:
XXXXX, which is hybridizable to SEQ ID NO:1;
(c) a polynucleotide encoding a polypeptide of SEQ ID NO:2 or the cDNA sequence included in ATCC Deposit No:
XXXXX, which is hybridizable to SEQ ID NO:1, having adiponectin binding activity;
(d) an isolated polynucleotide comprising nucleotides 213 to 1367 of SEQ ID NO:1, wherein said nucleotides encode a polypeptide comprising amino acids 2 to 386 of SEQ ID NO:2 minus the start methionine;
(e) an isolated polynucleotide comprising nucleotides 210 to 1367 of SEQ ID NO:1, wherein said nucleotides encode a polypeptide comprising amino acids 1 to 386 of SEQ ID NO:2 including the start codon;
(f) a polynucleotide which represents the complimentary sequence of SEQ ID NO:1;
(g) a polynucleotide of SEQ ID NO:3 or a polynucleotide of the cDNA sequence included in ATCC Deposit No:
XXXXX, which is hybridizable to SEQ ID NO:3;
(h) a polynucleotide encoding a polypeptide of SEQ ID NO:4 or a polypeptide encoded by the cDNA sequence included in ATCC Deposit No:
XXXXX, which is hybridizable to SEQ ID NO:3;
(i) a polynucleotide encoding a polypeptide of SEQ ID NO:4 or the cDNA sequence included in ATCC Deposit No:
XXXXX, which is hybridizable to SEQ ID NO:3, having adiponectin binding activity;
(j) an isolated polynucleotide comprising nucleotides 156 to 1310 of SEQ ID NO:3, wherein said nucleotides encode a polypeptide comprising amino acids 2 to 386 of SEQ ID NO:4 minus the start methionine;
(k) an isolated polynucleotide comprising nucleotides 153 to 1310 of SEQ ID NO:3, wherein said nucleotides encode a polypeptide comprising amino acids 1 to 386 of SEQ ID NO:4 including the start codon;
(l) a polynucleotide which represents the complimentary sequence of SEQ ID NO:3;
(m) a polynucleotide of SEQ ID NO:5 or a polynucleotide of the cDNA sequence included in ATCC Deposit No:
XXXXX, which is hybridizable to SEQ ID NO:5;
(n) a polynucleotide encoding a polypeptide of SEQ ID NO:6 or a polypeptide encoded by the cDNA sequence included in ATCC Deposit No:
XXXXX, which is hybridizable to SEQ ID NO:5;
(o) a polynucleotide encoding a polypeptide of SEQ ID NO:6 or the cDNA sequence included in ATCC Deposit No:
XXXXX, which is hybridizable to SEQ ID NO:5, having adiponectin binding activity;
(p) an isolated polynucleotide comprising nucleotides 4 to 864 of SEQ ID NO:5, wherein said nucleotides encode a polypeptide comprising amino acids 2 to 288 of SEQ ID NO:6 minus the start methionine;
(q) an isolated polynucleotide comprising nucleotides 1 to 864 of SEQ ID NO:5, wherein said nucleotides encode a polypeptide comprising amino acids 1 to 288 of SEQ ID NO:6 including the start codon;
(r) a polynucleotide which represents the complimentary sequence of SEQ ID NO:5;
(s) a polynucleotide of SEQ ID NO:101 or a polynucleotide of the cDNA sequence included in ATCC Deposit No:
XXXXX, which is hybridizable to SEQ ID NO:101;
(t) a polynucleotide encoding a polypeptide of SEQ ID NO:102 or a polypeptide encoded by the cDNA sequence included in ATCC Deposit No:
XXXXX, which is hybridizable to SEQ ID NO:101;
(u) a polynucleotide encoding a polypeptide of SEQ ID NO:102 or the cDNA sequence included in ATCC Deposit No:
XXXXX, which is hybridizable to SEQ ID NO:101, having adiponectin binding activity;
(v) an isolated polynucleotide comprising nucleotides 4 to 1143 of SEQ ID NO:101, wherein said nucleotides encode a polypeptide comprising amino acids 2 to 381 of SEQ ID NO:102 minus the start methionine;
(w) an isolated polynucleotide comprising nucleotides 1 to 1143 of SEQ ID NO:101, wherein said nucleotides encode a polypeptide comprising amino acids 1 to 381 of SEQ ID NO:102 including the start codon;
(x) a polynucleotide which represents the complimentary sequence of SEQ ID NO:101;
(y) a polynucleotide of SEQ ID NO:103 or a polynucleotide of the cDNA sequence included in ATCC Deposit No:
XXXXX, which is hybridizable to SEQ ID NO:103;
(z) a polynucleotide encoding a polypeptide of SEQ ID NO:104 or a polypeptide encoded by the cDNA sequence included in ATCC Deposit No:
XXXXX, which is hybridizable to SEQ ID NO:103;
(aa) a polynucleotide encoding a polypeptide of SEQ ID NO:104 or the cDNA sequence included in ATCC Deposit No:
XXXXX, which is hybridizable to SEQ ID NO:103, having adiponectin binding activity;
(bb) an isolated polynucleotide comprising nucleotides 210 to 1469 of SEQ ID NO:103, wherein said nucleotides encode a polypeptide comprising amino acids 2 to 421 of SEQ ID NO:104 minus the start methionine;
(cc) an isolated polynucleotide comprising nucleotides 207 to 1469 of SEQ ID NO:103, wherein said nucleotides encode a polypeptide comprising amino acids 1 to 421 of SEQ ID NO:104 including the start codon;
(dd) a polynucleotide which represents the complimentary sequence of SEQ ID NO:103;
(ee) a polynucleotide of SEQ ID NO:164 or a polynucleotide of the cDNA sequence included in ATCC Deposit No:
XXXXX, which is hybridizable to SEQ ID NO:164;
(ff) a polynucleotide encoding a polypeptide of SEQ ID NO:165 or a polypeptide encoded by the cDNA sequence included in ATCC Deposit No:
XXXXX, which is hybridizable to SEQ ID NO:164;
(gg) a polynucleotide encoding a polypeptide of SEQ ID NO:165 or the cDNA sequence included in ATCC Deposit No:
XXXXX, which is hybridizable to SEQ ID NO:164, having adiponectin binding activity;
(hh) an isolated polynucleotide comprising nucleotides 228 to 1349 of SEQ ID NO:164, wherein said nucleotides encode a polypeptide comprising amino acids 2 to 375 of SEQ ID NO:165 minus the start methionine;
(ii) an isolated polynucleotide comprising nucleotides 225 to 1349 of SEQ ID NO:164, wherein said nucleotides encode a polypeptide comprising amino acids 1 to 375 of SEQ ID NO:165 including the start codon;
(jj) a polynucleotide which represents the complimentary sequence of SEQ ID NO:164;
(kk) a polynucleotide of SEQ ID NO:166 or a polynucleotide of the cDNA sequence included in ATCC Deposit No:
XXXXX, which is hybridizable to SEQ ID NO:166;
(ll) a polynucleotide encoding a polypeptide of SEQ ID NO:167 or a polypeptide encoded by the cDNA sequence included in ATCC Deposit No:
XXXXX, which is hybridizable to SEQ ID NO:166;
(mm) a polynucleotide encoding a polypeptide of SEQ ID NO:167 or the cDNA sequence included in ATCC Deposit No:
XXXXX, which is hybridizable to SEQ ID NO:166, having adiponectin binding activity;
(nn) an isolated polynucleotide comprising nucleotides 133 to 1287 of SEQ ID NO:166, wherein said nucleotides encode a polypeptide comprising amino acids 2 to 386 of SEQ ID NO:167 minus the start methionine;
(oo) an isolated polynucleotide comprising nucleotides 130 to 1287 of SEQ ID NO:166, wherein said nucleotides encode a polypeptide comprising amino acids 1 to 386 of SEQ ID NO:167 including the start codon;
(pp) a polynucleotide which represents the complimentary sequence of SEQ ID NO:166;
and
(qq) a polynucleotide capable of hybridizing under stringent conditions to any one of the polynucleotides specified in (a)-(pp), wherein said polynucleotide does not hybridize under stringent conditions to a nucleic acid molecule having a nucleotide sequence of only A residues or of only T residues.

62 more elements

Polynucleotides encoding novel adiponectin receptor variants

Life of this patent

Polynucleotides encoding novel adiponectin receptor variants

Life of this patent

Abstract

Claims20