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US 2005/0032091 A1Published application
Method for improved specificity in probe based assays
AssigneeAdvanDx, Inc.
InventorsHenrik Stender, Thais Johansen
Life of this patent
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AssigneeAdvanDx, Inc.
InventorsHenrik Stender, Thais Johansen
Estimating the term…
Disclosed are compositions and methods for analyzing a target sequence in a sample. Generally, the method includes use of at least one pair of probes (Probe A and Probe B). In one embodiment, Probe A hybridizes to wanted and unwanted nucleic acid in the sample and bears a fluorophore and Probe B hybridizes to unwanted nucleic acid in the sample and bears a quencher. Fluorescence signal from Probe A hybridizing to unwanted nucleic acid is quenched by any relatively close hybridization of Probe B hereby increasing the specificity for the presence, amount or absence of the wanted target sequence. In preferred embodiments, the method is referred to as Fluorescence In-Situ Hybridization (FISH). The invention has many useful applications including rapidly detecting a microbial target sequence in a clinical sample.
A method for the analysis of a target sequence in a sample, said method comprising:
The method of claim 2, where the method is performed by fluorescence in situ hybridization.
The method of claim 1 or 13 where Probes A and B are labeled internally.
A kit suitable for performing an assay which detects the presence, absence or amount of target sequence in a sample, wherein said kit comprises a Probe A comprised of a nucleotide sequence, which hybridizes to a target region of both wanted and unwanted DNA or RNA and is labeled with a fluorophore at the end which, upon hybridization is closest to the adjacent target region for Probe B;
Dependencies are inferred from the claim text.