Method of attaching a biopolymer to a solid support
AssigneeDuke University
InventorsMichael Pirrung, Amy Odenbaugh, Richard Connors, Janice Worden
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Abstract
The present invention relates, in general, to a method of attaching a biopolymer to a solid support and, in particular, to a method of attaching a nucleic acid to a glass surface, and to reagents suitable for use in such a method. The invention further relates to the product produced by the present method and to kits comprising same.
Claims20
A method of functionalizing a solid support comprising contacting said support with a bromoacetamidosilane of Formula I
- wherein:
- R is an aryl or linear or branched alkyl,
- R1 and R2 are, independently, a linear or branched alkyl, linear or branched alkoxy, aryl or phenoxy, and
- X is a linker group, under conditions such that said bromoacetamidosilane reacts with groups present on said support.
A compound of the Formula I
- wherein:
- R is an aryl or linear or branched alkyl,
- R1 and R2 are, independently, a linear or branched alkyl, linear or branched alkoxy, aryl or phenoxy, and
- X is a linker group.
A solid support silanized with a bromoacetamidosilane of Formula I:
- wherein:
- R is an aryl or linear or branched alkyl,
- R1 and R2 are, independently, a linear or branched alkyl, linear or branched alkoxy, aryl or phenoxy, and
- X is a linker group.
A composition comprising a phosphorothioate-terminated biopolymer and a bromoacetamidosilylated solid support wherein said phosphorothioate-terminated biopolymer is bound to said support via a bromoacetamide group.
Dependencies are inferred from the claim text.