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US 2005/0032082 A1Published application
Methods for genotyping by hybridization analysis
AssigneeCambia
InventorAndrzej Kilian
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AssigneeCambia
InventorAndrzej Kilian
Estimating the term…
This invention provides methods for determining the genotype of organisms by hybridization analysis and, more specifically, to establishing the relatedness of individual organisms within a species. The present invention provides addressable arrays, comprising diversity panels of nucleic acid molecules, in which the molecules on the array are addressable or uniquely identifiable in some fashion. A diversity panel is the result of a method that can distinguish sequence differences between nucleic acid samples. As taught herein, a variety of methods may be used to generate diversity panels. Subsequent to the generation of the diversity panel, the nucleic acid products of the diversity panel are separated for application onto an array. The separated diversity panel is then delivered onto a substrate to create an addressable array and hybridized with labeled nucleic acids. The genotype of an organism is determined by the pattern of hybridization.
A method of genotyping, comprising the steps of:
A method of genotyping, comprising the steps of:
A method of genotyping, comprising the steps of:
A method of genotyping, comprising the steps of:
A method of identifying a nucleic acid molecule containing a polymorphism, comprising the steps of:
A method of identifying a nucleic acid molecule containing a polymorphism, comprising the steps of:
A kit for genotyping, comprising an addressable array of DNA molecules that comprises a diversity panel generated from nucleic acid molecules of two or more organisms, wherein the diversity panel comprises a reproducible pattern of nucleic acid molecules.
An ordered array of DNA molecules, wherein the DNA molecules are generated by a method comprising amplification of a mixture of nucleic acids isolated from two or more organisms.
An ordered array of DNA molecules, wherein the DNA molecules are generated by a method comprising restriction enzyme digestion with a methylation sensitive enzyme of a mixture of nucleic acids isolated from two or more organisms.
An ordered array of DNA molecules, wherein the DNA molecules are generated by a method comprising amplification of a mixture of nucleic acids isolated from two or more organisms, wherein the amplification uses a primer pair in which one primer anneals to a sequence that is conserved among a family of insertion elements.
Dependencies are inferred from the claim text.