Method and device for identifying substances capable of modulating adipocyte differentiation
AssigneeGenfit
InventorBart Staels
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Abstract
The invention concerns a method for identifying compounds capable of modulating adipocyte differentiation, which consists in contacting a compound to be tested with genetically modified pre-adipocyte cells overexpressing the REV-ERB ALPHA receptor and measuring the adipocyte differentiation of said genetically modified cells as compared with the adipocyte differentiation of same said genetically modified pre-adipocyte cells in the absence of said compound to be tested. The invention also concerns genetically modified pre-adipocyte cells overexpressing the REV-ERB ALPHA receptor as well as the method for preparing said cells.
Claims38
1-42. canceled.
A method for identifying compounds that modulates adipocyte differentiation, wherein (i) a test compound is contacted with a population of genetically modified pre-adipocyte cells comprising a recombinant nucleic acid coding a REV-ERB ALPHA receptor and (ii) adipocyte differentiation of said cells is measured or determined, allowing identification of compounds that modulate adipocyte differentiation.
A method for identifying compounds that modulate adipocyte differentiation, wherein said method comprises (i) contacting a test compound and a nucleic acid comprising sequence SEQ ID NO:
- 1 or a functional equivalent thereof, in the presence of the PPAR GAMMA receptor, (ii) verifying a binding of the PPAR GAMMA receptor to said nucleic acid, the test compounds modulating said binding of the PPAR GAMMA receptor being compounds modulating adipocyte differentiation.
A method for identifying compounds that modulate adipocyte differentiation, wherein said method comprises contacting a test compound and the PPAR GAMMA receptor with a reporter system comprising (i) a transcriptional promoter comprising one or more copies of sequence SEQ ID NO:
- 1 or a functional variant thereof and (ii) a reporter gene, and evaluating the activity of the test compound by measuring its effect on expression of the reporter gene induced by the PPAR GAMMA receptor.
A genetically modified pre-adipocyte cell, wherein said cell comprises a recombinant nucleic acid coding a REV-ERB ALPHA receptor, said recombinant nucleic acid further comprising sequence SEQ ID NO:
- 1 or a fragment thereof comprising sequence SEQ ID NO:
- 2.
A genetically modified pre-adipocyte cell, wherein said cell comprises a recombinant nucleic acid coding a REV-ERB ALPHA receptor, the recombinant nucleic acid being incorporated in a viral vector or integrated in the cellular genome.
A defective recombinant virus, wherein it comprises in its genome a nucleic acid coding a REV-ERB ALPHA receptor.
Dependencies are inferred from the claim text.