Process for preparing processed sample liquid solution for electrophoresis
AssigneeE.I. du Pont de Nemours and Company
InventorsRobert Kobos, Richard Reffuge, Robert Kachelries, Miriam Ronchi
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Abstract
A process for preparing a processed sample liquid solution for gel electrophoresis, comprising (a) treating a sample comprising a cell suspension in a non-shearing manner to produce a processed sample liquid solution comprising a mixture of DNA fragments extracted from the cell suspension, wherein at least one of the DNA fragments is greater than 200 kilobase pairs and (b) transferring the processed sample liquid solution in a non-shearing manner directly to an electrophoresis medium for conducting electrophoresis.
Claims28
A process for preparing a processed sample liquid solution for electrophoresis, comprising the steps of:
- (a) treating a sample comprising a cell suspension in a non-shearing manner to produce a processed sample liquid solution comprising a mixture of DNA fragments extracted from said cell suspension, wherein at least one of said DNA fragments is greater than 200 kilobase pairs;
- and
- (b) transferring said processed sample liquid solution in a non-shearing manner directly to an electrophoresis medium for conducting electrophoresis.
A process for separating a mixture of DNA fragments extracted from a cell suspension, comprising the steps of:
- (a) treating a sample comprising a cell suspension in a non-shearing manner to produce a processed sample liquid solution comprising a mixture of DNA fragments extracted from said cell suspension, wherein at least one of said DNA fragments is greater than 200 kilobase pairs;
- (b) transferring said processed sample liquid solution in a non-shearing manner directly to an electrophoresis medium;
- and
- (c) separating said mixture of DNA fragments by conducting electrophoresis.
Dependencies are inferred from the claim text.