Vitro growth of tissues suitable to the formation of bone and bone forming tissue formed thereby
AssigneeLifeNet
InventorsLloyd Wolfinbarger, XiaoFei Qin
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Abstract
The present invention is directed to a method of growing new bone, bone-like tissue or extracellular matrix under in vitro cell culture conditions. The method utilizes a bioreactor allowing for the flow of nutrient solutions into, through, and out of the bioreactor, wherein ground demineralized bone and bone-forming cells are present in the bioreactor. The resulting bone, bone-like tissue or extracellular matrix produced by the invention are within the scope of the present invention. In addition, the present invention is directed to the bioreactor device used to grow the new bone, bone-like tissue, or extracellular matrix.
Claims91
A method of growing new bone or bone-like tissue under in vitro cell culture conditions comprising providing ground demineralized bone and bone-forming cells in a bioreactor under conditions sufficient to form bone or bone-like biomaterial suitable for transplantation by causing a flow of nutrient solutions into, through, and out of the bioreactor.
A method for growing an extracellular matrix capable of forming bone when transplanted into a patient comprising providing bone-forming cells in a bioreactor under conditions sufficient to promote the growth and differentiation of cells into an extracellular matrix, wherein said conditions include the flow of nutrient solutions through the bioreactor.
A device for the growth of new bone or bone-like tissue under in vitro cell culture conditions comprising a bioreactor, wherein the bioreactor comprises an inlet port to receive nutrient solutions into the bioreactor, an outlet port to remove nutrient solutions from the bioreactor, sample injection ports, and an inlet port and outlet port for the bioreactor to cyclically receive negative pressure and positive pressure, optionally including hollow fibers for the delivery of nutrients and removal of wastes, wherein the bioreactor is capable of applying mechanical and electrical stimuli to the formed or forming new bone.
Dependencies are inferred from the claim text.