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US 2004/0210952 A1Published application
Transgenic animals
AssigneeAstellas Pharma Inc.
InventorsAkira Miyake, Yukihiro Nakamura, Jianwei Ni, Shinobu Mochizuki
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AssigneeAstellas Pharma Inc.
InventorsAkira Miyake, Yukihiro Nakamura, Jianwei Ni, Shinobu Mochizuki
Estimating the term…
There is discloses a transgenic mouse over-expressing a potassium channel BEC1, which can be used as an effective tool for screening a substance for antidementia or a substance to improve learning and memory. Also disclosed are an in vivo screening method of a substance for antidementia or a substance to improve learning and memory, which uses the learning and memory abilities of said mouse as the index, and an in vivo screening of a substance for antianxiety, which uses acceleration of anxiety as the index. In addition, there is disclosed a method for producing a pharmaceutical composition for antidementia, learning and memory improvement use or antianxiety, using a substance capable of inhibiting the learning and memory potassium channel activity as the active ingredient which can be obtained by the aforementioned screening method of the present invention.
A transgenic animal introduced with a sequence comprising a promoter and a polynucleotide encoding a potassium channel which is a polypeptide consisting of the amino acid sequence represented by SEQ ID NO:2 or a polypeptide comprising an amino acid sequence in which from 1 to 10 of amino acids in the amino acid sequence represented by SEQ ID NO:2 are deleted, substituted and/or added.
A transgenic animal which is a non-human animal or a offspring animal thereof obtained by effecting ontogenesis of a totipotent cell introduced, together with a promoter, with a polynucleotide encoding a potassium channel which is a polypeptide consisting of the amino acid sequence represented by SEQ ID NO:2 or a polypeptide comprising an amino acid sequence in which from 1 to 10 of amino acids in the amino acid sequence represented by SEQ ID NO:2 are deleted substituted and/or added, characterized in that the aforementioned polynucleotide is held on the chromosome and expresses said potassium channel in somatic cells.
A transgenic animal introduced with a sequence comprising a promoter and a polynucleotide encoding a polypeptide which consists of an amino acid sequence having a homology of 90% or more with the amino acid sequence represented by SEQ ID NO:2 and is a potassium channel.
A transgenic animal which is a non-human animal or a offspring animal thereof obtained by effecting ontogenesis of a totipotent cell introduced, together with a promoter, with a polynucleotide encoding a polypeptide which consists of an amino acid sequence having a homology of 90% or more with the amino acid sequence represented by SEQ ID NO:2 and is a potassium channel, characterized in that the aforementioned polynucleotide is held on the chromosome and expresses said potassium channel in somatic cells.
Dependencies are inferred from the claim text.