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US 2004/0204569 A1Published application
Separation material, column having that separation material, and use thereof for purifying proteins
OwnerEdmund Radmacher, Klaus Moller +1inventors
InventorsEdmund Radmacher, Klaus Moller, Helmut Riering
Life of this patent
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Abstract
The invention concerns a separation matrix for purifying His-tag proteins by the introduction of alkyl substituents into a TED molecule, if applicable by the use of support materials having an average pore width >10−7 m (1000 Å).
Claims16
A separation matrix for purifying His-tag proteins that contains a porous support on which a chelating group is bound according to the general formula I below:
- 5
- where R1 is a branched or unbranched alkyl group containing 1 to 20 carbon atoms, an aralkyl group containing 1 to 20 carbon atoms, an aryl group containing 1 to 20 carbon atoms, or a heteroaryl group containing 1 to 20 carbon atoms as well as at least one of the elements N, S, O, P;
- R2, R3, and R4 are identical or different and represent hydrogen, branched or unbranched alkyl groups containing 1 to 20 carbon atoms, aralkyl groups containing 1 to 20 carbon atoms, and/or aryl groups containing 6 to 18 ring atoms;
- and
- the support has an average pore width larger than 10−7 m (1000 Å).
2 more elements
A separation matrix for purifying His-tag proteins that contains a porous support on which a chelating group is bound according to the general formula II below:
- 6
- where R1 is a branched or unbranched alkyl group containing 1 to 20 carbon atoms, an aralkyl group containing 1 to 20 carbon atoms, an aryl group containing 1 to 20 carbon atoms, or a heteroaryl group containing 1 to 20 carbon atoms as well as at least one of the elements N, S, O, P;
- and
- R2, R3, and R4 are identical or different and represent hydrogen, branched or unbranched alkyl groups containing 1 to 20 carbon atoms, aralkyl groups containing 1 to 20 carbon atoms, and/or aryl groups containing 6 to 18 ring atoms, with the stipulation that no more than two of the groups R2, R3, and R4 are present as hydrogen.
1 more element
Dependencies are inferred from the claim text.