Support for cell/tissue culture and culture method
AssigneeMebiol Inc.
InventorsHiroshi Yoshioka, Sunao Kubota, Yuichi Mori
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Abstract
A cell or tissue-culturing carrier which can effectively regenerate an intended a cell or tissue, while suppressing an excessive growth of fibroblasts, and a method of culturing a cell or tissue by using the above carrier, the cell or tissue-culturing carrier wherein fibroblasts showing substantially no growing property in a gel based on the hydrogel-forming polymer, is constituted by using a hydrogel-forming polymer; an aqueous solution of which shows a thermo-reversible sol-gel transition such that it assumes a sol state at a lower temperature and assumes a gel state at a higher temperature.
Claims8
A cell or tissue-culturing carrier, comprising, at least a hydrogel-forming polymer;
- an aqueous solution of the hydrogel-forming polymer showing a thermo-reversible sol-gel transition such that it assumes a sol state at a lower temperature and assumes a gel state at a higher temperature;
- fibroblasts showing substantially no growing property in a gel based on the hydrogel-forming polymer.
A method of culturing a cell or tissue, comprising:
- providing a cell or tissue-culturing carrier comprising, at least a hydrogel-forming polymer;
- an aqueous solution of the hydrogel-forming polymer showing a thermo-reversible sol-gel transition such that it assumes a sol state at a lower temperature and assumes a gel state at a higher temperature;
- fibroblasts showing substantially no growing property in a gel based on the hydrogel-forming polymer;
- adding a cell or tissue to the carrier assuming a sol state at a temperature lower than the sol-gel transition temperature thereof;
- culturing the cell or tissue by using the carrier assuming a gel state at a temperature higher than the sol-gel transition temperature thereof;
- and recovering the cultured cell or tissue by converting again the carrier into a sol state at a temperature lower than the sol-gel transition temperature thereof.
Dependencies are inferred from the claim text.