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US 2004/0203140 A1Published application
Interlinked culture chamber for biologicals
AssigneeSynthecon, Inc.
InventorsRoger Akers, William J. Anderson, Stephen S. Navran JR.
Life of this patent
Estimating the term…
Abstract
The bioreactor system of the present invention has two fluid-filled culture compartments in which cells, tissues and other biologicals are cultured. The two culture compartments are in fluid communication with each other and each culture compartment is transversed by a filter that prevents the exit of the cells from the culture compartment. Both reusable and disposable culture chambers are described for use as the first and/or second culture compartment.
Claims55
A culture system comprising:
- (a) a fluid inlet;
- (b) a first culture compartment having a tubular housing;
- (c) a first end piece attached to the fluid inlet on one side and to a first end of the tubular housing on a second side,
- (d) a second culture compartment coaxial with the first culture compartment, the second culture compartment having a proximal end and a distal end;
- (e) a fluid connector having a first side mounted on a second end of the tubular housing and a second side mounted on the proximal end of the second culture compartment, the fluid connector having a through bore passing from the first side to the second side of the fluid connector;
- (f) a connector filter positioned on the first side of the fluid connector to filter a fluid stream passing out of the first culture compartment and into the through bore of the fluid connector;
- (g) a fluid outlet;
- (h) a distal end piece mounted on the distal end of the second culture compartment and connected to the fluid outlet;
- and
- (i) an outlet filter supported by the distal end piece.
7 more elements
A culture system comprising:
- (a) a fluid inlet;
- (b) a first culture compartment having
- (i) a tubular sleeve,
- (ii) a growth compartment within the sleeve,
- (iii) a first end piece attached to the fluid inlet on one side and to a first end of the tubular housing on a second side, and
- (iv) a membrane carrier assembly transversing the growth compartment comprising
- a support cylinder,
- a molecular weight cut-off membrane secured to an exterior surface of the support cylinder, and
- a chamber between the exterior surface of the cylinder and an interior surface of the membrane, the chamber in fluid communication with the fluid inlet and the growth compartment;
- (c) a second culture compartment coaxial with the first culture compartment, the second culture compartment having a proximal end and a distal end;
- (d) a fluid connector having a first side mounted on a second end of the tubular sleeve and a second side mounted on the proximal end of the second culture compartment, the fluid connector having a through bore passing from the first side to the second side of the fluid connector wherein the through bore is in fluid communication with the chamber of the membrane carrier assembly and the interior of the second culture compartment;
- (e) a fluid outlet;
- (f) a distal end piece mounted on the distal end of the second culture compartment and connected to the fluid outlet;
- and
- (g) an outlet filter supported by the distal end piece.
12 more elements
A culture system comprising:
- (a) a fluid inlet;
- (b) a first culture compartment having a tubular housing;
- (c) a first end piece attached to the fluid inlet on one side and to a first end of the tubular housing on a second side,
- (d) a second culture compartment coaxial with the first culture compartment, the second culture compartment having a proximal end and a distal end;
- (e) a fluid connector having a first side mounted on a second end of the tubular housing and a second side mounted on the proximal end of the second culture compartment, the fluid connector having a through bore passing from the first side to the second side of the fluid connector;
- (f) a connector filter positioned on the first side of the fluid connector to filter a fluid stream passing out of the first culture compartment and into the through bore of the fluid connector;
- (g) a fluid outlet;
- (h) a distal end piece mounted on the distal end of the second culture compartment and connected to the fluid outlet;
- and
- (i) an outlet filter transversing the second culture compartment including:
- a support cylinder having a first end supported by the fluid connector and a second end supported by the distal end piece,
- a molecular weight cut-off membrane secured to an exterior surface of the support cylinder, and
- a chamber between the exterior surface of the cylinder and an interior surface of the membrane, the chamber in fluid communication with the through bore of the fluid connector and the fluid outlet.
10 more elements
A culture system comprising:
- (a) a fluid inlet;
- (b) a first culture compartment having a tubular housing;
- (c) a first end piece attached to the fluid inlet on one side and to a first end of the tubular housing on a second side,
- (d) a fluid connector having a first side mounted on a second end of the tubular housing, the fluid connector having a through bore passing from the first side to a second side of the fluid connector;
- (e) a connector filter positioned on the first side of the fluid connector to filter a fluid stream passing out of the first culture compartment and into the through bore of the fluid connector;
- (f) a culture bag including
- a flexible external wall having a first end, a second end, an internal side, and an external side, wherein the internal side of the external wall is positioned to face an interior of the culture bag,
- a first bag end fused to the first end of the external wall and attached to the second side of the fluid connector,
- a second bag end fused to the second end of the external wall, and
- an outlet filter supported by the second bag end;
- and
- (g) a fluid outlet.
9 more elements
A culture system comprising:
- (a) a fluid inlet;
- (b) a first culture bag having
- a flexible external wall having a first end, a second end, an internal side, and an external side, wherein the internal side of the external wall is positioned to face an interior of the first culture bag,
- a first bag end fused to the first end of the external wall and attached to the fluid inlet,
- a second bag end fused to the second end of the external wall, and
- a first bag filter positioned on the second bag end to filter a fluid stream passing out of the first culture bag;
- (c) a fluid connector having a first side mounted on the second bag end, the fluid connector having a through bore passing from the first side to a second side of the fluid connector;
- (d) a second culture bag including
- a flexible outer wall having a first end, a second end, an internal side, and an external side, wherein the internal side of the outer wall is positioned to face an interior of the second culture bag,
- a proximal bag end fused to the first end of the outer wall and attached to the second side of the fluid connector,
- a distal bag end fused to the second end of the outer wall, and
- an outlet filter supported by the distal bag end;
- and
- (g) a fluid outlet.
11 more elements
Dependencies are inferred from the claim text.