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AssigneeDomantis Limited
InventorsRudolf Maria Theodora de Wildt, Laurent Jespers, Ian Michael Tomlinson
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Herein, immunoglobulin variable region polypeptides are fused at their N-terminus to a bacterial signal sequence. In one embodiment, the immunoglobulin variable region polypeptides are fused to a tag that that is placed in between a bacteriophage signal sequence and the N-terminus of the immunoglobulin variable region polypeptide. In another embodiment, the N-termini of single-domain antibodies (dAb) are fused to a bacterial signal sequence in the absence of a tag. In a further embodiment, the immunoglobulin variable region antibody fusion proteins are fused to a bacteriophage coat protein and expressed on the surface of bacteriophage providing an efficient way to select small antibody fragments that are labeled and that have high affinity to target ligand.
A nucleic acid molecule comprising a first DNA sequence encoding the signal peptide of a bacteriophage protein linked at its 3′ end to a second DNA sequence encoding a tag wherein the second DNA sequence is linked at its 3′ end to a third DNA sequence encoding an immunoglobulin variable region polypeptide.
A polypeptide molecule comprising a first amino acid sequence comprising a signal peptide of a bacteriophage protein that is linked at it's C-terminus to the N-terminus of a second amino acid sequence comprising a tag, wherein the second amino acid sequence is linked at its C-terminus to a third amino acid sequence comprising an immunoglobulin variable region polypeptide.
A nucleic acid vector comprising a DNA sequence encoding a signal peptide of a bacteriophage protein linked at its 3′ end to a second DNA sequence encoding a tag, which is in turn linked at its 3′ end to a third DNA sequence encoding an immunoglobulin variable region polypeptide.
Dependencies are inferred from the claim text.