Method for evaluating uniformity of spots in arrays

Abstract

A method whereby the uniformity of spots on a DNA microarray is evaluated by extremely simple means. A method for evaluating a uniformity of spots on a DNA microarray having a plurality of spots, these spots undergoing specific emissions as a result of the hybridization of target DNA and tagged probe DNA, wherein the uniformity of the spots is evaluated by examining whether patterns having periodicity are manifested in a sequence BG comprising background data obtained in a manner described below. <Method for Preparing the Sequence BG> (1) By applying an analysis software to images obtained by scanning the monochromatic emission of the DNA microarray, background data for each spot are obtained. (2) Concerning each spot, the corresponding plate No.(α) of the target DNA and position (β, Γ) are determined, wherein a is a symbol or a numerical symbol identifying the plate while β and Γ respectively stand for the row and column of a matrix formed by plate holes. (3) The No.(α) and the position (β, Γ) on the plate are assigned to respective background data. (4) The sequence BG consisting of the background data aligned in the orders of the plate No.(α) (the first priority) and the position (β, Γ) on the plate(the second priority) is obtained.

Dependencies are inferred from the claim text.