Capillary electrophoresis apparatus
AssigneeHitachi High-Technologies Corporation
InventorsTomoyuki Sakai, Takashi Anazawa
Life of this patent
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Abstract
A capillary array electrophoresis which can measure fluorescence of a number of capillaries at a time with high sensitivity and can automatically analyze a sample sequentially. An end edge as a sample dissolution edge of a number of capillaries is connected to a polymer filling block having a mechanism of filling the polymer as an isolation medium inside a capillary. Fluorescence irradiated from an edge surface of an end edge portion of the capillary is detected through a detection window in which distance between an external surface and a detection surface is smaller than focal distance of an optical lens closest to a detection flat surface.
Claims20
A capillary array electrophoresis apparatus, comprising:
- a capillary for filling a polymer;
- a polymer container being provided in a vicinity of one end edge of said capillary;
- a polymer injector for injecting said polymer into said polymer container and said capillary via said polymer container;
- a sample container being provided in a vicinity of another end edge of said capillary;
- voltage applying means for applying voltage between said one end edge and said another end portion of said capillary;
- an irradiator for irradiating said capillary;
- and
- fluorescence detection means for detecting fluorescence emitted from a sample via said polymer container.
A capillary array electrophoresis apparatus, comprising:
- a capillary for filling a polymer;
- a polymer container being provided in a vicinity of one end edge of said capillary;
- a polymer injector for injecting said polymer into said polymer container and said capillary via said polymer container;
- a sample container being provided in a vicinity of another end edge of said capillary;
- voltage applying means for applying voltage between said one end edge and said another end edge of said capillary;
- an irradiator for irradiating said capillary;
- and
- fluorescence detection means for detecting fluorescence emitted from a sample,
- wherein said polymer is introduced and discharged essentially in a vertical direction to a moving direction of said polymer inside said capillary in said polymer container.
A capillary array electrophoresis method, comprising:
- filling a polymer inside said capillary from one end edge of capillary being connected with a polymer container;
- performing electrophoretic process of a sample toward said one end edge of said capillary from another end edge portion of said capillary;
- and
- detecting fluorescence emitted by laser irradiation to said sample through said polymer container.
Dependencies are inferred from the claim text.