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AssigneeUniversity of Florida Research Foundation, Inc.
InventorsZhijian T. Li, Dennis J. Gray
Estimating the term…
The subject invention pertains to the isolation of nucleotide sequences of the 2S albumin gene and its promoter from grape. Promoter sequences of the invention provide for seed-specific transcription of operably linked polynucleotide sequences. Seed-specific expression activity of the subject promoter was also characterized by using transient green fluorescent protein (GFP) expression analysis in transformed somatic embryos (SE) of grape (cv. Thompson Seedless). The subject invention also concerns expression constructs comprising a promoter of the present invention. Plants, plant tissues, and plant cells bred to contain or transformed with a polynucleotide of the invention are also contemplated by the present invention.
An isolated polynucleotide comprising a promoter sequence, or a fragment thereof, from a 2S albumin gene of grape.
An expression construct comprising at least one promoter sequence, or a fragment thereof, from a 2S albumin gene of grape.
A cell transformed with a polynucleotide comprising a promoter sequence, or a fragment thereof, from a 2S albumin gene of grape.
A transformed or transgenic plant, or the progeny thereof, comprising a promoter sequence, or a fragment thereof, from a 2S albumin gene of grape.
An isolated polynucleotide comprising a nucleotide sequence encoding a 2S albumin protein of grape.
A method for promoting seed-specific transcription of a polynucleotide, said method comprising transforming a plant cell with a nucleic acid comprising a promoter sequence, or a fragment thereof, from a 2S albumin gene of grape operably linked with said polynucleotide, and growing a plant or plant tissue from said transformed cell.
A polynucleotide probe or primer that selectively hybridizes with a nucleotide, sequence of a 2S albumin gene.
Dependencies are inferred from the claim text.