Genes encoding sulfate assimilation proteins
AssigneeE. I. Du Pont de Nemours and Company
InventorsSaverio Carl Falco, Stephen M. Allen, Shawn L. Anderson
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Abstract
This invention relates to an isolated nucleic acid fragment encoding a sulfate assimilation protein. The invention also relates to the construction of a chimeric gene encoding all or a portion of the sulfate assimilation protein, in sense or antisense orientation, wherein expression of the chimeric gene results in production of altered levels of the sulfate assimilation protein in a transformed host cell.
Claims11
An isolated nucleic acid fragment encoding an APS kinase comprising a member selected from the group consisting of:
- (a) an isolated nucleic acid fragment encoding an amino acid sequence that is at least 80% identical to the amino acid sequence set forth in a member selected from the group consisting of SEQ ID NO:2, 4, 6, 8, 10 and 12
- (b) an isolated nucleic acid fragment that is complementary to (a).
An APS kinase polypeptide comprising all or a substantial portion of the amino acid sequence set forth in a member selected from the group consisting of SEQ ID NO:2, 4, 6, 8, 10 and 12.
A method of obtaining a nucleic acid fragment encoding a substantial portion of an amino acid sequence encoding a sulfate assimilation protein comprising:
- (a) synthesizing an oligonucleotide primer corresponding to a portion of the sequence set forth in any of SEQ ID NOs:1, 3, 5, 7, 9, and 11;
- and
- (b) amplifying a cDNA insert present in a cloning vector using the oligonucleotide primer of step (a) and a primer representing sequences of the cloning vector
- wherein the amplified nucleic acid fragment encodes a substantial portion of an amino acid sequence encoding a sulfate assimilation protein.
Dependencies are inferred from the claim text.