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OwnerTony Mikaer Wahlroos, Josif Grigorievieh Atabekov +4inventors
InventorsTony Mikaer Wahlroos, Josif Grigorievieh Atabekov, Yurii Leonidivich Dorokhov, Petri Eerik Susi, Mauri Jaakko Makela, Timo Kalevi Korpela
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The present invention provides constructs and methods for increasing the content of selected amino acids by targeted expression or accumulation of a protein enriched with an amino acid sequence entailing said protein in a plant species or in a tissue or an organ of a plant. The increased content is obtained by stable transformation of plants with a recombinant nucleotide sequence construct encoding a carrier protein having in its 3′-terminal end a polyamino acid extension and operably linked with a tissue or an organ specific regulatory sequence. The increased amino acid content in plant tissues, particularly membraneous oil bodies and cell walls of seed provides a composition useful as animal feed.
A method for increasing the content of one or more selected amino acids in a selected tissue or organ of a plant comprising transformation of a plant with at least one recombinant nucleotide sequence construct comprising tissue or organ specific regulatory sequences driving transcription during selected stages of morphogenesis, the construct being operably linked to a chimeric nucleotide sequence comprising:
A recombinant nucleotide sequence construct for increasing the content of one or more selected amino acids in a selected tissue or organ of a plant, wherein the construct which enables stable targeted expression of an amino acid-enriched protein having a polyamino acid extension in a selected tissue or organ of a plant comprises a tissue or organ specific regulatory sequence driving transcription during selected stages of morphogenesis operably linked to a chimeric nucleotide sequence comprising;
Dependencies are inferred from the claim text.