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US 2004/0176596 A1Published application
Vitamin b6-phosphate phosphatase
AssigneeDsm IP Assets B.V.
InventorsTatsuo Hoshino, Keiko Ichikawa, Masaaki Tazoe
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Abstract
Vitamin B6-phosphate phosphatase (VB6PP), a process for producing VB6PP and a process for producing vitamin B6 from vitamin B6-phosphate (VB6P) utilizing VB6PP and cell-free extract of a specific microorganism capable of producing VB6PP.
Claims15
A vitamin B6 phosphate-phosphatase having the following physico-chemical properties:
- a) Molecular weight:
- 29,000±5,000 (consisting of a monomer having a molecular weight of 29,000±5,000)
- b) Co-factor:
- Mn2+, Mg2+, Co2+, Sn2+ or Ni2+
- c) Substrate specificity:
- active on pyridoxol 5′-phosphate, pyridoxal 5′-phosphate and pyridoxamine 5′-phosphate
- d) Optimum temperature:
- 30-40° C. at pH 7.5
- e) Optimum pH:
- 7.0-8.0
7 more elements
Dependencies are inferred from the claim text.