Fluorescent dye binding peptides
AssigneeThe Board of Trustees of the Leland Stanford Junior University
InventorsGarry P. Nolan, Michael N. Rozinov
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Abstract
The present invention is directed to novel polypeptides, termed fluorettes, that bind with high avidity to fluorophore dyes. The peptides find use in a variety of methods and approaches involving fluorophore dyes.
Claims28
A peptide comprising a sequence of at least about 8 amino acids, wherein said peptide binds to a fluorophore dye.
A complex comprising a fluorophore dye and a peptide comprising a sequence of at least about 8 amino acids, wherein said dye is bound by said peptide.
A method of binding a peptide to a fluorophore dye, comprising;
- contacting said fluorophore dye with said peptide, wherein said peptide comprises a sequence of at least about 8 amino acids that binds said fluorophore dye.
A method of detecting a fluorette comprising
- a) contacting a fluorophore dye and fluorette comprising at least 8 amino acids under conditions wherein said dye and said fluorette bind to form a dye complex;
- and
- b) detecting the presence of said dye complex.
A peptide comprising an amino acid sequence, X1—X2—X3—X4—Y—W-T-X5-M-F—Y—X6, wherein,
- X1 is selected from the group consisting of K, N and T;
- X2 is selected from the group consisting of H, P, and N;
- X3 is selected from the group consisting of A and V;
- X4 is selected from the group consisting of H and Q;
- X5 is selected from the group consisting of H and Q;
- X6 is selected from the group consisting of S and T.
A peptide comprising an amino acid sequence, X1—P—H—X2—P-M-Y—W-T-X3—V—F, wherein,
- X1 is selected from the group consisting of I and L;
- X2 is selected from the group consisting of P and R;
- X3 is selected from the group consisting of P and R.
A peptide comprising an amino acid sequence, X1—X2—W—X3—Y—X4—W-D-W-T-X5-F—W,
- X1 is selected from the group consisting of H and Q;
- X2 is selected from the group consisting of E and G;
- X3 is selected from the group consisting of D and E;
- X4 is selected from the group consisting of Y and N;
- X5 is selected from the group consisting of A, D, P, and T;
A peptide comprising an amino acid sequence, Y—X1—X2—X3—X4—X5—W—W—X6—Y—Y—X7, wherein,
- X1 is selected from the group consisting of H, P, and T, or is optionally omitted;
- X2 is selected from the group consisting of H, N, S, and Y;
- X3 is selected from the group consisting of D and E;
- X4 is selected from the group consisting of F and Y;
- X5 is selected from the group consisting of D and E;
- X6 is selected from the group consisting of D and E;
- X7 is selected from the group consisting of F, L, M, and Y.
Dependencies are inferred from the claim text.