Conopeptides and methods of use
OwnerFrank Mari, Gregg B. Fieldsinventors
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Abstract
Disclosed are methods and compositions for modulation of ion levels in cells. The compositions contain a new class of biologically active peptides derived from the venom of predatory marine snails of the species Conus gladiator and Conus mus. The active peptides, termed γ-Hydroxyconophans are heavily hydroxylated small peptides. These peptides contain a definitive structural motif which is a double modification of the polypeptide chain in contiguous residues, i.e., γ-OH-Hyv-D-Trp.
Claims20
An isolated γ-Hydroxyconophan peptide, said peptide comprising the amino acids γ-OH-Val and D-Trp in contiguous residues.
An isolated peptide comprising the amino acid sequence of SEQ ID NO:2.
An isolated peptide comprising the amino acid sequence of SEQ ID NO:3.
An isolated peptide comprising the amino acid sequence A-O-X1-N-S-X2-W-S (SEQ ID NO:1) wherein:
- O is γ-hydroxyproline;
- X1 is A or S;
- X2 is V or γ-hydroxyvaline (V*);
- and
- W is D-Tryptophan.
A method of modulating the level of an ion within a cell, the method comprising the steps of:
- (a) providing a cell that responds to a peptide that binds to a chemical structure on the surface of said cell by modulating the level of at least one ion within said cell;
- and
- (b) contacting said chemical structure on the surface of said cell with a peptide comprising the amino acids γ-OH-Val and D-Trp in contiguous residues, wherein said peptide selectively binds to said chemical structure.
A method of modulating the level of an ion within a cell, the method comprising the steps of:
- (a) providing a cell that responds to a peptide that binds to a chemical structure on the surface of said cell by modulating the level of at least one ion within said cell;
- and
- (b) contacting said chemical structure on the surface of said cell with a peptide comprising the amino acids A-O-X1-N-S-X2-W-S (SEQ ID NO:1) in contiguous residues, wherein said peptide selectively binds to said chemical structure, wherein:
- O is γ-hydroxyproline;
- X1 is A or S;
- X2 is V or γ-hydroxyvaline (V*);
- and
- W is D-Tryptophan.
Dependencies are inferred from the claim text.