Indolicidin analogs with anti-microbial activity
AssigneeCouncil of Scientific and Industrial Research
InventorsChilukuri Subbalakshmi, E. Bikshapathy, Narasimhaiah Sitaram, Ramakrishnan Nagaraj
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Abstract
The present invention relates to three single tryptophan analogs ILPWKLPLLPLRR-amide (IL4), ILPLKLPWLPLRR-amide (IL8) and ILPLKLPLLPWRR-amide (IL11), of indolicidin, a cationic tridecapeptide amide found in the granules of bovine neutrophils, said analogs having said amino acid only at 4th, 8th or 11th position from N-terminal, with leucine at its all other conventional positions, said analogs selectively having only anti-microbial activity and no hemolytic activity of Indolicidin, and thereby providing therapeutic options and a method thereof.
Claims22
Three single tryptophan analogs of Indolicidin having amino-acid sequence of ILPWKLPLLPLRR-amide (IL4), ILPLKLPWLPLRR-amide (IL8) and ILPLKLPLLPWRR-amide (IL11), with said amino acid at positions 4, 8 and 11 respectively from N-terminal.
A method of producing three single tryptophan analogs of Indolicidin having amino-acid sequence of ILPWKLPLLPLRR-amide (IL4), ILPLKLPWLPLRR-amide (IL8) and ILPLKLPLLPWRR-amide (IL11), with said amino acid at positions 4, 8 and 11 respectively from N-terminal, said method comprising:
- (i) synthesizing peptide sequence of said analogs with C-terminal amide, and
- (ii) purifying syntheized sequence using conventional method.
A composition useful for treating anti-microbial infections, said composition comprising one or more single tryptophan analogs of Indolicidin having amino-acid sequence of ILPWKLPLLPLRR-amide (IL4), ILPLKLPWLPLRR-amide (IL8) and ILPLKLPLLPWRR-amide (IL11), with said amino acid at positions 4, 8 and 11 respectively from N-terminal and optionally pharmaceutically acceptable additives.
A method of treating anti-microbial infections using composition comprising one or more single typtophan analogs of Indolicidin having amino-acid sequence of ILPWKLPLLPLRR-amide (IL4), ILPLKLPWLPLRR-amide (IL8) and ILPLKLPLLPWRR-amide (IL11), with said amino acid at positions 4, 8 and 11 respectively from N-terminal and optionally pharmaceutically acceptable additives, said method comprising steps of adminstering said composition to a subject.
Dependencies are inferred from the claim text.