Methods for identifying compounds for regulating muscle mass or function using vasoactive intestinal peptide receptors
AssigneeThe Procter & Gamble Company
InventorsRobert Joseph Isfort, Russell James Sheldon
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Abstract
Screening methods for identifying compounds that regulate skeletal muscle atrophy or hypertrophy, regulate the activity or expression of the vasoactive intestinal peptide receptors (VPAC) or regulate expression of vasoactive intestinal peptide (VIP) or VIP analogs are provided. Methods for the prophylactic or therapeutic treatment of skeletal muscle atrophy utilizing VPAC as the target for intervention are described.
Claims24
A method for identifying candidate compounds for regulating skeletal muscle mass or function, comprising:
- a. contacting a test compound with a VPAC receptor;
- b. determining whether the test compound binds to the VPAC receptor;
- and
- c. identifying those test compounds that bind VPAC receptor as candidate compounds for regulating skeletal muscle mass or function.
A method for identifying candidate compounds for regulating skeletal muscle mass or function, comprising:
- a. contacting a test compound with a VPAC receptor;
- b. determining whether the test compound binds to the VPAC receptor;
- c. selecting the compounds that bind the VPAC receptor and further determining whether the test compound modulates muscle mass or function in a skeletal muscle atrophy model system;
- and
- d. identifying those test compounds that modulate muscle mass or function in a skeletal muscle atrophy model system as candidate compounds for regulating skeletal muscle mass or function.
A method for identifying candidate compounds for regulating skeletal muscle mass or function comprising:
- a. contacting a test compound with a cell expressing a functional VPAC2 receptor, and determining level of activation of VPAC2 receptor resulting from the interaction of the test compound with VPAC2 receptor;
- b. contacting the test compound with a cell expressing a functional VPAC1 receptor, and determining level of activation of VPAC1 receptor resulting from the interaction of the test compound with VPAC1 receptor;
- c. comparing the level of VPAC2 receptor activation with the level of VPAC1 receptor activation;
- and
- d. identifying those test compounds that selectively activate VPAC2 receptor over the VPAC1 receptor as candidate compounds for regulating skeletal muscle mass or function.
Dependencies are inferred from the claim text.