Method for calibrating mass spectrometers with trityl mass-tags
OwnerMikhail Sergeevich Shchepinov, Edwin Mellor Southerninventors
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Abstract
The subject invention relates to processes of measuring molecular mass by mass spectrometry, in particular to methods of precise calibration of mass spectrometeres, and to kits and systems for use in calibrating mass spectrometeres.
Claims37
A method of measuring the molecular mass of a compound Y of unknown molecular mass by mass spectrometry, comprising
- providing a sample of compound Y,
- providing samples of at least two different compounds each of Formula (I), R—X in which R is a trityl group and X is cleavable to form a charged species for mass spectrometry,
- and recording the molecular mass of compound Y and the at least two compounds of Formula (I) in a mass spectrometer.
Use of a compound of Formula (I) R—X in which R is a trityl group and X is cleavable to form a charged species for mass spectrometry as a calibration compound for mass spectrometry.
A kit for the production of calibration compounds for mass spectrometry comprising:
- (a) at least one base reactant of Formula (I) R—X where R is a trityl group and X is cleavable to form a charged species for mass spectrometry;
- and
- (b) at least two different amine compounds which are of different molecular masses and which are each capable of reacting with the base reactant;
- wherein the base reactant (a) is packaged separately from amine compounds (b).
A set of calibration compounds for mass spectrometry comprising at least two separately packaged mixtures (a) and (b), wherein
- mixture (a) comprises at least two different compounds each of formula (I) R—X and having different molecular masses, and
- mixture (b) comprises at least two further compounds of formula (I) R—X having different molecular masses and wherein R is a trityl group and X is cleavable to form a charged species for mass spectrometry.
A kit for the production of a set of calibration compounds comprising a first package comprising a base reactant of Formula (I) R—X, in which R is a trityl group and X is cleavable to form a charged species for mass spectrometry, and at least two separate second packages (a) and (b), each containing a mixture of at least two amine compounds which have different molecular masses and which are capable of reacting with the base reactant.
A method of measuring the molecular mass of a compound Y of unknown molecular mass comprising
- estimating the expected molecular mass of compound Y, selecting at least one calibration compound of Formula (I) R—X having molecular weight close to the expected molecular weight of the compound Y, in which R is a trityl group and X is cleavable to form a charged species for mass spectrometry
- and subjecting both compounds to mass spectrometry simultaneously.
A mixture of at least two compounds of Formula (I) R—X in which R is a trityl group and X is a group cleavable to give a charged species for analysis by mass spectrometry.
A method of mass spectrometry comprising subjecting simultaneously to mass spectrometry at least two different compounds of Formula (I) R—X in which R is a trityl group and X is cleavable to give a charged species for analysis by mass spectrometry.
Dependencies are inferred from the claim text.