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US 2004/0175830 A1Published application
Phagemid vectors
AssigneeAlexion Pharmaceuticals, Inc.
InventorsKatherine S. Bowdish, Shana Frederickson, Martha Wild
Life of this patent
Estimating the term…
Abstract
Phagemid vectors containing a sequence of features between a Col E1 origin and an f1 origin are useful for display of polypeptides or proteins, including antibody libraries.
Claims9
A phagemid vector comprising:
- a selectable marker;
- a ColE1 origin;
- an f1 origin;
- and
- after the ColE1 origin but before the f1 origin, further comprising the following features:
- a bacterial transcription terminator;
- a promoter;
- a first ribosomal binding site;
- a first leader sequence;
- a first cloning region;
- a second ribosomal binding site;
- a second leader sequence;
- a second cloning region for receiving a gene encoding a polypeptide to be displayed;
- and
- a nucleotide sequence encoding a product that enables display of a polypeptide on the surface of a phagemid particle.
12 more elements
A phagemid vector comprising Seq. ID No. 18.
A vector comprising a sequence selected from the group consisting of Seq. ID Nos. 19, 20 and 21.
Dependencies are inferred from the claim text.