Cultured cartilage/bone cells/tissue, method of generating same and uses thereof
AssigneeTechnion Research & Development Foundation Limited
InventorGila Maor
Life of this patent
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Abstract
A method of generating cultured chondrocytes/endochondral bone cells is disclosed. The method comprises isolating chondrocytes from mandibular condyle tissue, and culturing said isolated chondrocytes.
Claims107
A method of generating cultured chondrocytes, the method comprising:
- (a) isolating chondrocytes from mandibular condyle tissue;
- and
- (b) culturing said isolated chondrocytes, thereby generating cultured chondrocytes.
A method of generating cultured endochondral bone cells, the method comprising:
- (a) isolating chondrocytes from mandibular condyle tissue;
- and
- (b) culturing said isolated chondrocytes under conditions suitable for formation of endochondral bone cells, thereby generating cultured endochondral bone cells.
A method of redifferentiating dedifferentiated chondrocytes, the method comprising culturing dedifferentiated chondrocytes under culturing conditions which comprise a culture medium including at least one supplement selected from the group consisting of ascorbic acid, beta-glycerophosphate, pyruvate and IGF-I, said culturing conditions being devoid of a three dimensional support and/or of a biomolecule-coated support, thereby redifferentiating said dedifferentiated chondrocytes.
Isolated mandibular condyle tissue comprising chondrocytes and being depleted of fibroblast-like cells and/or myocytes.
A cell culture comprising isolated chondrocytes being capable of generating endochondral bone cells when cultured under culturing conditions which:
- (i) include a two dimensional support not coated with a biomolecule;
- and
- (ii) a culture medium devoid of a supplement selected from the group consisting of a microfilament-modifying compound, a protein kinase inhibitor and a polypeptide growth factor, said supplement not being derived from a serum supplement of said culture medium.
A method of treating a cartilage or bone disease in a subject, the method comprising:
- (a) isolating chondrocytes from mandibular condyle tissue;
- (b) culturing said isolated chondrocytes, thereby generating cultured chondrocytes;
- and
- (c) administering a therapeutically effective dose of said cultured chondrocytes to the subject, thereby treating the cartilage or bone disease in the subject.
A method of isolating chondrocytes from mandibular condyle tissue, the method comprising:
- (a) isolating mandibular condyle tissue from a mammal and treating the mandibular condyle tissue so as to selectively remove fibroblast-like cells and/or myocytes therefrom, thereby generating modified mandibular condyle tissue depleted of said fibroblast-like cells and/or said myocytes, said modified mandibular condyle tissue including chondrocytes;
- and
- (b) selectively harvesting said chondrocytes from said modified mandibular condyle tissue, thereby isolating chondrocytes from mandibular condyle tissue.
Dependencies are inferred from the claim text.