# US 2004/0175823 A1 · Isolation of spore-like cells from tissues exposed to extreme conditions > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 2004/0175823 A1 - Document ID: US20040175823A1 - Status: Published application - Kind: A1 - Application number: 10/792,302 - Filed: 2004-03-03 - Published: 2004-09-09 - Earliest claimed: 2000-10-30 - Assignees: VBI Technologies, L.L.C. - Inventors: Charles A. Vacanti; Martin P. Vacanti - Web page: https://patentgrep.com/patent/US20040175823A1 ## CPC - C12N 5/0602: {Vertebrate cells} - A61K 35/12: Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells (vaccines or medicinal preparations containing antigens or antibodies A61K39/00) - C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00) - A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D) ## Abstract Highly undifferentiated spore-like cells can be isolated from many different tissues and bodily fluids after those tissues and fluids have been exposed to extreme conditions. The spore-like cells can be used to treat a wide variety of disorders. ## Claims ### Claim 1 A method for isolating a spore-like cell from a biological tissue or fluid, the method comprising (a) obtaining a tissue or fluid that has been exposed to an environment in which differentiated or partially differentiated cells in the tissue or fluid die and (b) separating the spore-like cells from the differentiated or partially differentiated cells that have died. ### Claim 2 (depends on 1) The method of claim 1, further comprising disrupting the tissue or fluid either before or after step (a). ### Claim 3 (depends on 2) The method of claim 2, wherein disrupting the tissue or fluid comprises cutting the tissue into pieces, scraping the tissue with a blunt instrument, or passing the tissue or fluid through a series of devices having progressively smaller apertures. ### Claim 4 (depends on 3) The method of claim 3, wherein the devices are pipettes. ### Claim 5 (depends on 4) The method of claim 4, wherein the smallest pipette has an inner bore diameter of approximately 15 microns. ### Claim 6 (depends on 3) The method of claim 3, wherein the devices are filters. ### Claim 7 (depends on 6) The method of claim 6, wherein the finest filter has a pore size of approximately 15 microns. ### Claim 8 (depends on 1) The method of claim 1, wherein the biological tissue comprises a tissue that originates from the endoderm. ### Claim 9 (depends on 1) The method of claim 1, wherein the biological tissue comprises a tissue that originates from the mesoderm. ### Claim 10 (depends on 1) The method of claim 1, wherein the biological tissue comprises a tissue that originates from the ectoderm. ### Claim 11 (depends on 1) The method of claim 1, wherein the biological fluid comprises blood, urine, or saliva. ### Claim 12 (depends on 1) The method of claim 1, wherein the biological fluid is cerebrospinal fluid. ### Claim 13 (depends on 1) The method of claim 1, wherein the environment is an oxygen-poor environment. ### Claim 14 (depends on 1) The method of claim 1, wherein the environment is one in which the temperature is above or below the range of temperatures in which differentiated or partially differentiated cells can survive. ### Claim 15 (depends on 1) The method of claim 1, wherein the environment contains a toxin or infectious agent that kills differentiated or partially differentiated cells. ### Claim 16 A method for isolating a spore-like cell from a biological tissue or fluid, the method comprising obtaining a tissue or fluid that has been exposed to an environment that is either 42° C., or more, or 0° C., or less, placing the tissue or fluid in a tissue culture vessel, allowing the spore-like cells to adhere to the vessel, and rinsing away non-spore-like cells, the tissue or fluid having been exposed to the environment without first being treated with a protective agent. ### Claim 17 (depends on 16) The method of claim 16, further comprising disrupting the tissue or fluid either before or after exposure to the environment or before or after placement in the tissue culture vessel. ### Claim 18 (depends on 17) The method of claim 17, wherein disrupting the tissue comprises cutting the tissue into pieces, scraping the tissue with a blunt instrument, or passing the tissue or fluid through a series of devices having progressively smaller apertures. ### Claim 19 (depends on 18) The method of claim 18, wherein the devices are pipettes. ### Claim 20 (depends on 14) The method of claim 14, wherein the smallest pipette has an inner bore diameter of approximately 15 microns. ### Claim 21 (depends on 18) The method of claim 18, wherein the devices are filters. ### Claim 22 (depends on 21) The method of claim 21, wherein the finest filter has a pore size of approximately 15 microns. ### Claim 23 (depends on 16) The method of claim 16, wherein the biological tissue or fluid originates from the endoderm. ### Claim 24 (depends on 16) The method of claim 16, wherein the biological tissue or fluid originates from the mesoderm. ### Claim 25 (depends on 16) The method of claim 16, wherein the biological tissue is a tissue that originates from the ectoderm. ### Claim 26 (depends on 16) The method of claim 16, wherein the biological fluid is blood, urine, or saliva. ### Claim 27 (depends on 16) The method of claim 16, wherein the biological fluid is cerebrospinal fluid. ### Claim 28 (depends on 16) The method of claim 16, wherein the environment is 0° C. or less and the protective agent is a cryopreservative. ### Claim 29 (depends on 16) The method of claim 16, wherein the tissue or fluid is intentionally exposed to the environment.