Method for cloning and expression of OkrAI restriction endonuclease and methyltransferase
AssigneeNew England Biolabs, Inc.
InventorsShuang-Yong Xu, Jian-Ping Xiao, Rebecca B. Kucera, James Samuelson
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Abstract
The present invention relates to recombinant DNA encoding the OkrAI restriction endonuclease as well as OkrAI methylase, expression of OkrAI restriction endonuclease in E. coli cells containing the recombinant DNA.
Claims6
Isolated DNA encoding the OkrAI restriction endonuclease, wherein the isolated DNA is obtainable from Oceanospirillum kriegii.
A cloning vector comprising a vector into which a DNA segment encoding the OkrAI restriction endonuclease has been inserted.
Isolated DNA encoding the OkrAI restriction endonuclease, wherein the isolated DNA is obtainable from ATCC No. PTA-5002.
Dependencies are inferred from the claim text.