Method for fixing biomolecules onto chemically inert surfaces

A process as claimed in any of the preceding claims, characterized in that the reactive functional group is a carboxyl, amino, hydroxy and/or thio group, optionally in activated, more particularly protonated or deprotonated form,

A process as claimed in any of the preceding claims, characterized in that the activation of the chemically inert carrier surface by plasmachemical methods in step (a) is selectively carried out on the surface only.

A process as claimed in any of the preceding claims, characterized in that, during the activation of the chemically inert carrier surface by plasmachemical methods in step (a), the bulk properties of that surface remain otherwise intact.

A process as claimed in any of the preceding claims, characterized in that the activation of the chemically inert carrier surface in step (a) is carried out in a reactive plasma, more particularly a high-frequency plasma.

A process as claimed in any of the preceding claims, characterized in that the chemically inert carrier surface comprises noble metals, such as in particular platinum and alloys thereof, stainless steel or polyhalogenated polymers, more particularly polyhalogenated polymeric hydrocarbons, such as in particular polytetrafluoroethylene or polyvinyl chloride, or cellulose acetate or combinations of these materials.

A process as claimed in any of the preceding claims, characterized in that, in step (b), the binding or coupling of the biomolecules to be immobilized onto the plasmachemically modified carrier surface is carried out by binding or coupling of the biomolecules via the functional groups reactive in step (a).

A process as claimed in any of the preceding claims, characterized in that the biomolecules are directly or indirectly bound to the carrier surface by covalent and/or ionic bonding, preferably covalent bonding, via the reactive functional group(s).

A process as claimed in any of the preceding claims, characterized in that the biomolecule to be immobilized is an enzyme and is selected in particular from the group consisting of oxidoreductases, transferases, hydrolases such as, in particular, esterases such as lipases, lyases, isomerases and ligases (synthetases) and mixtures or combinations thereof.

A process as claimed in any of the preceding claims, characterized in that process step (b) may be followed by a process step (c) comprising crosslinking of the biomolecules bound or coupled to the chemically inert carrier surface in process step (b), process steps (b) and (c) optionally being carried out in combination and, more particularly, at the same time.

An immobilized biomolecule, more particularly enzyme or enzymatic system, characterized in that the biomolecule is directly or indirectly fixed, more particularly bound or coupled, to a chemically inert carrier surface, the biomolecule being bound or coupled to the chemically inert carrier surface via suitable reactive functional groups applied to that surface.

A biosensor, more particularly for the qualitative and/or quantitative determination of peroxides, more particularly organic and/or inorganic peroxides, such as hydrogen peroxide;

• dissolved peroxides from inorganic or organic salts;
• and/or organic peracids, the biosensor comprising at least one peroxide-sensitive biomolecule, more particularly enzyme, preferably catalase, fixed to an activated, plasmachemically modified and/or functionalized carrier surface of a chemically inert carrier material, preferably polytetrafluoroethylene.

A biosensor, more particularly for the qualitative and/or quantitative determination of glucose, more particularly β-D-glucose, the biosensor comprising at least one peroxide-sensitive biomolecule, more particularly enzyme, preferably glucoseoxidase, optionally in combination with catalase, fixed to an activated, plasmachemically modified and/or functionalized carrier surface of a chemically inert carrier material, preferably polytetrafluoroethylene.