Increasing bioavailability of carotenoids
OwnerJoseph Kanner, Rina Granit +1inventors
InventorsJoseph Kanner, Rina Granit, Arieh Levy
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Abstract
A method of increasing a fraction of free carotenoids in a source of carotenoids in which at least some of the carotenoids are fatty acid esterified carotenoids is disclosed. The method is effected by contacting the source of carotenoids with an effective amount of an esterase under conditions effective in deesterifying the fatty acid esterified carotenoids, thereby increasing the fraction of free carotenoids in the source of carotenoids.
Claims107
A method of determining an efficiency of an esterase in increasing a fraction of free carotenoids in a source of carotenoids in which at least some of the carotenoids are fatty acid esterified carotenoids, the method comprising:
- contacting the source of carotenoids with the esterase under preselected experimental conditions;
- and
- using a carotenoids detection assay for determining the efficiency of the esterase in increasing the fraction of the free carotenoids in the source of carotenoids.
A method of screening for esterases efficient in increasing a fraction of free carotenoids in a source of carotenoids in which at least some of the carotenoids are fatty acid esterified carotenoids, the method comprising:
- contacting the source of carotenoids separately with each of the esterases under preselected experimental conditions;
- and
- using a carotenoids detection assay for determining the efficiency of each of the esterases in increasing the fraction of the free carotenoids in the source of carotenoids, thereby screening for esterases efficient in increasing the fraction of free carotenoids in the source of carotenoids.
A method of optimizing reaction conditions for increasing a fraction of free carotenoids in a source of carotenoids in which at least some of the carotenoids are fatty acid esterified carotenoids, via an esterase, the method comprising:
- contacting the source of carotenoids with the esterase under different preselected experimental conditions;
- and
- using a carotenoids detection assay for determining the efficiency of the esterase in increasing the fraction of the free carotenoids in the source of carotenoids under each of said different preselected experimental conditions, thereby optimizing the reaction conditions for increasing the fraction of free carotenoids in the source of carotenoids in which at least some of the carotenoids are fatty acid esterified carotenoids via the esterase.
A method of increasing a fraction of free carotenoids in a source of carotenoids in which at least some of the carotenoids are fatty acid esterified carotenoids, the method comprising contacting the source of carotenoids with an effective amount of an esterase under conditions effective in deesterifying the fatty acid esterified carotenoids, thereby increasing the fraction of free carotenoids in the source of carotenoids.
A method of extracting red pepper oleoresin, the method comprising:
- homogenizing red-pepper fruits in water into a juice;
- centrifuging the juice so as to obtain a pellet;
- mixing the pellet with ethanol and ethyl acetate;
- homogenizing the pellet with the ethanol and the ethyl acetate;
- removing dry material;
- and
- evaporating solvents so as to obtain red pepper oleoresin.
Dependencies are inferred from the claim text.